FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699549
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33690.19822905959169168No Hit
ATATTATGCAGCGTCCGTGCAGGGGCGGTTCTCCATGTCCAGAGACAATT24760.14568570838498918No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20950.12326799639198399No Hit
GCATAATATGTATTGGTGCCACTGTCACTAATACCTGAGACCCACTCCAG17340.10202706718076383No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38250.075.105451
TCAACGC46800.060.004954
ATCAACG47000.059.7496153
TATCAAC50600.055.9255642
CAACGCA52250.053.8838735
AACGCAG54650.051.517526
CGCAGAG60050.046.5250748
GGTATCA28100.041.5086561
GCAGAGT68400.040.9423189
ACGCAGA70050.039.986177
TATACCG1352.2456465E-532.0026445
ATACCGT1952.770139E-729.54096
CAGAGTA67600.028.9668399
GTACATG48900.027.5335851
TACATGG48650.027.38152
ACATGGG50050.026.4717183
CATGGGG32450.023.9649984
GTATACG1650.002708335621.8180561
GTCTTAT6600.020.7271541
GAATACG3157.7980803E-720.568899
Produced by FastQC (version 0.11.5)