FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697872
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA50190.7191863264323544No Hit
GTCTATTACTGTGCGAGAGAGTGGGATTTTTGGAGTGGTTGGACGGATTA42130.6036923676548135No Hit
GTAGTAATCCGTCCAACCACTCCAAAAATCCCACTCTCTCGCACAGTAAT19180.2748354999197561No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC18300.26222573767109153No Hit
GATTACTACTACTATTACGGTTTGGACGTCTGGGGCCAAGGGACCACGGT17680.2533415869958961No Hit
TTAGTAGACTCATCCAAGAACCAACTCTCCCTGAGGCTGAGCTCTGTGAC16390.23485682188137655No Hit
GTCTACTAATATGATGACTCGACCCTCGAGGGCGGGATTGTATTGGGCGC15790.22625925671183253No Hit
CTATTACGGTTTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCGT13130.18814338446018755No Hit
CCGTAATAGTAGTAGTAATCCGTCCAACCACTCCAAAAATCCCACTCTCT13100.18771350620171035No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAGACGATTAGTA12900.1848476511451957No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA12490.17897264827934065No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC12330.1766799642341289No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12200.17481715844739437No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12030.1723811816493569No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA11990.17180801063805395No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11990.17180801063805395No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11810.16922874108719077No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11750.16836898457023639No Hit
ATACAATCCCGCCCTCGAGGGTCGAGTCATCATATTAGTAGACTCATCCA11660.1670793497948048No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAG11620.16650617878350182No Hit
GTATTGGGCGCTTCCACTATGATTGATTCTACCAATCCACTCCAGTCCCT11320.16220739619872984No Hit
GTCTCTGGTGGGTCCCTCAGAAGTTATCACTGGAACTGGATCCGCCAGCC11240.16106105417612399No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11230.16091776142329825No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11030.1580519063667836No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10940.156762271591352No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10920.15647568608570053No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC10870.15575922232157188No Hit
ACTCAGGACCCTGCTGTGTCGGTGGCCTTGGGACAGACAGTCAGGATCAC10840.15532934406309468No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA10760.1541830020404888No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10670.1528933672650572No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10460.1498842194557168No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT9940.14243299630877868No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG9930.14228970355595294No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC9650.13827750647683243No Hit
GAATAGGGGCCTGCCCTGGCTTCTGCTGGTACCAGCTTGGATAATCGTTT9580.13727445720705228No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9360.13412201664488616No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9300.13326226012793177No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG9180.13154274709402297No Hit
GGTATGTTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCGGTGTCTCTGG8930.12796042827337964No Hit
CTACTAATCGTCTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT8880.127243964509251No Hit
GCCTGAGGTGGAGCCAGAGAATCGGTCCGGGATCTCTGAGGGCCGGTTGT8710.12480798771121351No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8570.12280188917165326No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8490.12165554714904739No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC8390.12022261962079006No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8350.11964944860948713No Hit
GTCCCAGGTGCAGCTACAGCAATGGGGCGCAGGTATGTTGAAGCCTTCGG8210.11764335006992686No Hit
TAGTGGAAGCGCCCAATACAATCCCGCCCTCGAGGGTCGAGTCATCATAT8200.11750005731710114No Hit
CCCTCATGCTGCCACCCCCGACTGTCACTGCACCGACCGGCCCTCGAGGA8190.1173567645642754No Hit
CCCCTATTCTTGTCATCTATGGTCAAAACAACCGGCCCTCAGAGATCCCG8130.116497008047321No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTC8030.11506408051906367No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC7990.11449090950776074No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAGACGGTGACCGTGGTCCCTTGG7810.11191163995689754No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCT7700.11033541967581446No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG7690.11019212692298874No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTGTTTGCATAGCATCTG7630.10933237040603434No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC7630.10933237040603434No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA7570.10847261388907996No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC7570.10847261388907996No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7540.10804273563060275No Hit
CAATAATAGTCAGCCTCATCGTCCGCCTGAGCCCCAGTGATGGTCAAGGA7510.10761285737212553No Hit
GTCCCTCACCTGCGGTGTCTCTGGTGGGTCCCTCAGAAGTTATCACTGGA7500.10746956461929981No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7470.10703968636082262No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA7380.10575005158539103No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7350.10532017332691382No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7310.10474700231561088No Hit
CTATTACTGTGCGAGAGAGTGGGATTTTTGGAGTGGTTGGACGGATTACT7310.10474700231561088No Hit
CTATTATTGTCATTCTCGGGACATCACTACTAATCGTCTGGTGTTCGGCG7300.10460370956278514No Hit
GTACATGGGAGTGCTTTCTGAGAGTCATGGACCTCCTGCACAAGAACATG7280.10431712405713368No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGA7230.10360066029300502No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7170.10274090377605062No Hit
TAGTAGTAGTAATCCGTCCAACCACTCCAAAAATCCCACTCTCTCGCACA7150.10245431827039916No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAGACGATTAGTAG7030.10073480523649035No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG7010.1004482197308389No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6990.10016163422518742No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG657.580758E-444.317915
TATCAAC11950.043.396982
ATCAACG11800.043.335133
TCAACGC11850.043.1522834
CAACGCA12650.040.4203875
GTATCAA12900.040.206851
AACGCAG13150.038.877916
CAGCCGT19450.037.0185629
ACGCAGA14100.036.769167
ACAGCCG19950.036.451698
CGCAGAG14150.036.1277668
AATAGAC20500.035.835223
TACATGG20050.035.5644232
TAGACAG20500.035.481355
ATAGACA21750.035.100264
GTACATG20600.034.619851
CCTATAC1251.3231773E-534.570453
TAATAGA22100.033.5691152
CTGTGCG18450.033.5614939
TATTGAG8600.033.4960945
Produced by FastQC (version 0.11.5)