FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713519
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAGACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATATA47540.6662751797779737No Hit
GTCTACTACTGTGCGAGCGACTGGCACGCCGGATACTGGGGCCAGGGAAC38990.5464465557329238No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17990.2521306370257835No Hit
TTTAAGAGGTGTCCTGTGTCAGGTGCACCTGGAGGAGTCTGGGGGAGGCG17960.2517101857133447No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17860.25030868133854883No Hit
GTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGCAGTGGGTGGCCGTTA17380.24358146033952843No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15510.217373328530845No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15020.21050595709434508No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13030.18261602003590655No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12970.181775117411029No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12480.17490774597452907No Hit
TTCATATACTATGAGTGAGAGGTACTACGCAGACTCCGTGCAGGGCCGAT12380.17350624159973316No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12120.1698623302252638No Hit
TACTATGAGTGAGAGGTACTACGCAGACTCCGTGCAGGGCCGATTCACCA11790.1652373657884373No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11740.16453661360103936No Hit
GCTATGTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGCAGTGGGTGGC11720.16425631272608018No Hit
GTACTACGCAGACTCCGTGCAGGGCCGATTCACCATCTCCAGAGACAAGT11530.16159345441396797No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10890.15262382641527417No Hit
GATTTATAGTATAAGGAACAAACACTCCTGGACCCCTGCCCGGTTCTCAG10560.14799886197844767No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10180.14267314535422324No Hit
CTATGAGTGAGAGGTACTACGCAGACTCCGTGCAGGGCCGATTCACCATC10110.14169209229186608No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9330.13076035816845802No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9160.12837780073130498No Hit
CACTATATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTCTAC9150.1282376502938254No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9060.1269762963565091No Hit
CTATAAATCAGTGTCCTGGGTGCTTGTCCAGGCTTCTGCTGGATCCAGTG8990.12599524329415193No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8900.12473388935683562No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8900.12473388935683562No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAGAGCTG8860.12417328760691726No Hit
GTCTGGGGGAGGCGTGGTCCGGCCTGGGGGGTCCCTGAGACTCTCCTGTG8750.12263163279464177No Hit
GTATATGAAATAACGGCCACCCACTGCAGCCCCTTGCCTGGAGCCTGGCG8470.11870742054521323No Hit
CTCCTGTGCAGCCTCTGGATTCAACTTCAATGACTTTGCTATGTACTGGG8360.11716576573293773No Hit
CCATAGTAGAGCAGGCAGTAATAGTCCGCCTCGTCCTCAGGCTGCACACC8250.11562411092066224No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8250.11562411092066224No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG8240.11548396048318266No Hit
GTACATAGCAAAGTCATTGAAGTTGAATCCAGAGGCTGCACAGGAGAGTC8240.11548396048318266No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAACTTCAATGACTTTGCT8230.11534381004570306No Hit
CTCATAGTATATGAAATAACGGCCACCCACTGCAGCCCCTTGCCTGGAGC8210.11506350917074389No Hit
GATATAGTGTGTTCTTGGACTTGTCTCTGGAGATGGTGAATCGGCCCTGC8150.11422260654586633No Hit
GACTTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCACGACACCACCA8130.11394230567090716No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8030.11254080129611124No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8030.11254080129611124No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGGAGTCGCCGGTGTT8010.11226050042115207No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC7970.1116998986712337No Hit
GTCCTGTGTCAGGTGCACCTGGAGGAGTCTGGGGGAGGCGTGGTCCGGCC7910.11085899604635617No Hit
GTCCAAGAACACACTATATCTGCAAATGAACAGCCTGAGAGCTGAGGACA7800.10931734123408067No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC7750.1086165890466827No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC7740.10847643860920311No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7660.10735523510936638No Hit
GCGTAGTACCTCTCACTCATAGTATATGAAATAACGGCCACCCACTGCAG7460.10455222635977457No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT7410.10385147417237663No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC7310.10244996979758073No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT7300.10230981936010114No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT7290.10216966892262155No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT7270.10188936804766237No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)