FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713519
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAGACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATATA46730.6549229943421269No Hit
GTCTACTACTGTGCGAGCGACTGGCACGCCGGATACTGGGGCCAGGGAAC38530.5399996356088625No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20380.28562659158340564No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17130.2400776994025387No Hit
TTTAAGAGGTGTCCTGTGTCAGGTGCACCTGGAGGAGTCTGGGGGAGGCG17040.23881634546522237No Hit
GTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGCAGTGGGTGGCCGTTA16980.2379754428403448No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15180.21274836409401854No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14680.205740842220039No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13580.19032429409728402No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13220.18527887834801876No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13000.18219556872346776No Hit
TTCATATACTATGAGTGAGAGGTACTACGCAGACTCCGTGCAGGGCCGAT11990.1680403745380291No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11800.1653775162259169No Hit
GATTTATAGTATAAGGAACAAACACTCCTGGACCCCTGCCCGGTTCTCAG11800.1653775162259169No Hit
GTACTACGCAGACTCCGTGCAGGGCCGATTCACCATCTCCAGAGACAAGT11180.15668818910218227No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11010.15430563166502925No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10890.15262382641527417No Hit
TACTATGAGTGAGAGGTACTACGCAGACTCCGTGCAGGGCCGATTCACCA10860.15220337510283538No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10500.14715795935357012No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10410.14589660541625382No Hit
GCTATGTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGCAGTGGGTGGC10140.14211254360430486No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10030.14057088879202936No Hit
CTATGAGTGAGAGGTACTACGCAGACTCCGTGCAGGGCCGATTCACCATC9980.1398701366046314No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9480.1328626147306519No Hit
CTCCTGTGCAGCCTCTGGATTCAACTTCAATGACTTTGCTATGTACTGGG9190.12879825204374376No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAGAGCTG9180.12865810160626417No Hit
GTCTGGGGGAGGCGTGGTCCGGCCTGGGGGGTCCCTGAGACTCTCCTGTG9100.12753689810642743No Hit
GTATATGAAATAACGGCCACCCACTGCAGCCCCTTGCCTGGAGCCTGGCG9040.12669599548154992No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9000.12613539373163152No Hit
CTATAAATCAGTGTCCTGGGTGCTTGTCCAGGCTTCTGCTGGATCCAGTG8820.12361268585699892No Hit
GATATAGTGTGTTCTTGGACTTGTCTCTGGAGATGGTGAATCGGCCCTGC8780.12305208410708053No Hit
CACTATATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTCTAC8690.12179073016976422No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT8560.11996877448252954No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC8500.119127871857652No Hit
GTACATAGCAAAGTCATTGAAGTTGAATCCAGAGGCTGCACAGGAGAGTC8490.1189877214201724No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8480.11884757098269282No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC8110.11366200479594799No Hit
CCATAGTAGAGCAGGCAGTAATAGTCCGCCTCGTCCTCAGGCTGCACACC8010.11226050042115207No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT7970.1116998986712337No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG7940.11127944735879493No Hit
GACTTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCACGACACCACCA7910.11085899604635617No Hit
CTCATAGTATATGAAATAACGGCCACCCACTGCAGCCCCTTGCCTGGAGC7890.11057869517139698No Hit
GTCCTGTGTCAGGTGCACCTGGAGGAGTCTGGGGGAGGCGTGGTCCGGCC7760.1087567394841623No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAACTTCAATGACTTTGCT7750.1086165890466827No Hit
GCGTAGTACCTCTCACTCATAGTATATGAAATAACGGCCACCCACTGCAG7700.10791583685928477No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7500.10511282810969295No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7500.10511282810969295No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7490.10497267767221335No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG7470.10469237679725417No Hit
CTACTATGGTGGTGTCGTGGTTTTCGGCGGAGGGACCAGGCTGACCGTCC7210.10104846542278483No Hit
GTCCAAGAACACACTATATCTGCAAATGAACAGCCTGAGAGCTGAGGACA7190.10076816454782564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)