FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170711
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA58140.4966212839889605No Hit
GTGTATTACTGTGCGAGAGATCTTAGCACCAGGTGGGCACGGCCACTGGA41970.358500090970359No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCGGGGGGAGGCG31360.26787140464213627No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23380.19970769899659266No Hit
GTATATCATATGATGGAAGTAATAAATATTACGCAGACTCCGTGAAGGGC22920.19577846283156133No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22850.19518053558905657No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22250.1900554449390157No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21560.18416159069146867No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGCCTGGAGTGGGTGGC20470.17485100934389444No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20000.1708363550013624No Hit
ATATTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT19630.16767588243383721No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18970.16203828271879225No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17850.15247144683871594No Hit
TTCCAAGACCACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACA17500.14948181062619212No Hit
GTATTACTGTGCGAGAGATCTTAGCACCAGGTGGGCACGGCCACTGGACT16780.14333170184614308No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16770.1432462836686424No Hit
CTCCAGGCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGCTAC16720.142819192781139No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA15510.13248359330355655No Hit
ATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAGCG15480.13222733877105453No Hit
GTATTACTGTCAGCAGTATGGTAGTTCACGTCCGATCACTTTCGGCCCTG15470.1321419205935538No Hit
ATGATATACCTGCCACCCACTCCAGGCCCTTGCCTGGAGCCTGGCGGACC15230.1300918843335375No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15190.12975021162353476No Hit
GTCTTGGAATTGTCTCTGGAGATGGTGAATCGGCCCTTCACGGAGTCTGC15080.12881061167102725No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14910.1273585026535157No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT14860.12693141176601228No Hit
GCGTAATATTTATTACTTCCATCATATGATATACCTGCCACCCACTCCAG14600.12471053915099456No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13890.11864584854844619No Hit
GGCCTGGAGTGGGTGGCAGGTATATCATATGATGGAAGTAATAAATATTA13770.11762083041843803No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC13450.11488744873841623No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT13340.11394784878590873No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13090.1118123943483917No Hit
TCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC13070.11164155799339033No Hit
TCTTAGCACCAGGTGGGCACGGCCACTGGACTACTGGGGCCAGGGAACCC12960.11070195804088286No Hit
GCTGTGTATTACTGTGCGAGAGATCTTAGCACCAGGTGGGCACGGCCACT12500.1067727218758515No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGG12380.10574770374584333No Hit
GGTATATCATATGATGGAAGTAATAAATATTACGCAGACTCCGTGAAGGG12310.10514977650333858No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG12300.10506435832583788No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12220.10438101290583245No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12110.10344141295332493No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12040.10284348571082018No Hit
GTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACT11900.10164763122581064No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11840.10113512216080656No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18700.073.620951
GGTATCA7100.069.033551
ATCAACG20850.064.3035663
TATCAAC23050.058.4788552
TCAACGC23950.055.980354
AACGCAG24550.055.1923456
CAACGCA25250.053.6691445
CGCAGAG27550.048.8747068
GCAGAGT33000.041.015929
TGTACGC1301.7217815E-533.268722
GTACATG29500.032.49671
ACATGGG29300.032.227893
TACATGG29750.031.4981162
CAGCGCT7450.030.9244699
CAGCCGT31800.030.7907549
GAATACG1703.1805448E-629.6454229
TACACCG1952.743327E-729.5721995
CATGGGA16650.029.4389864
TACACAG39850.028.0370035
GTAATAC42000.026.6006371
Produced by FastQC (version 0.11.5)