FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837360
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATACACAGCCGTGTCTTCTGGTCTCAGGCTGCTCATTTGCAGATATA28830.3442963599885354No Hit
CAATAATACACAGCCGTGTCTTCTGGTCTCAGGCTGCTCATTTGCAGATA21680.2589089519442056No Hit
GTGTATTATTGTGTGAGAGATGGTTGGTCCCTCTGGGGCCAGGGAACCCT20830.24875800133753703No Hit
GTATTATTGTGTGAGAGATGGTTGGTCCCTCTGGGGCCAGGGAACCCTGG20400.24362281456004586No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19760.235979745867966No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18880.22547052641635618No Hit
CCCTATATCTGCAAATGAGCAGCCTGAGACCAGAAGACACGGCTGTGTAT18860.22523168051972867No Hit
GCTTTACACTGGGTCCGCCAGGCTCCGGGCAAGGGGCTGGAGTGGGTGGC17590.21006496608388267No Hit
GATATAGGGTGTTCCTGGAATTGTCCCTGGAGACGGTGAATCGGCCCCTC17540.20946785134231394No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17380.20755708416929397No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16910.2019442055985478No Hit
ACTAAATACTATTCAGACTCCGTGAGGGGCCGATTCACCGTCTCCAGGGA16800.20063055316709658No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16120.19250979268176172No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14670.17519346517626827No Hit
CTATATCATATCTTGAAGCTACTAAATACTATTCAGACTCCGTGAGGGGC14490.17304385210662082No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14440.17244673736505206No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14360.17149135377854208No Hit
TTTAGGAGGTGTCCGGTGTCAAGTGGACCTGGTGGAGTCGGGGGGAGGCG14250.17017770134709087No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14120.16862520301901213No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13730.16396770803477598No Hit
GTGTAAAGCATAGTCACTGAAGATGAATCCAGAGGCTGCACAGGAGACTC12860.1535779115314799No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12840.1533390656348524No Hit
CTATAATGGTTATTCCAGTTTTGGCCAGGGGACCAAGCTGGAGATGAAAC12840.1533390656348524No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12290.14677080347759625No Hit
GCACACAGAGGACTCACCATGGAGTTTGGGCTGACCTGGGTTTTCCTCGT12160.14521830514951753No Hit
GGGCTGGAGTGGGTGGCAACTATATCATATCTTGAAGCTACTAAATACTA11770.14056081016528135No Hit
GTATTTAGTAGCTTCAAGATATGATATAGTTGCCACCCACTCCAGCCCCT11550.1379335053023789No Hit
ATGATATAGTTGCCACCCACTCCAGCCCCTTGCCCGGAGCCTGGCGGACC11150.13315658736982897No Hit
CCATTATAGCGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGGCT11110.132678895576574No Hit
ATACTATTCAGACTCCGTGAGGGGCCGATTCACCGTCTCCAGGGACAATT10760.12849909238559282No Hit
GATCAGCACTGCACACAGAGGACTCACCATGGAGTTTGGGCTGACCTGGG10540.12587178752269038No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT10360.1237221744530429No Hit
ATATCATATCTTGAAGCTACTAAATACTATTCAGACTCCGTGAGGGGCCG10240.12228909907327791No Hit
CTCCAGCCCCTTGCCCGGAGCCTGGCGGACCCAGTGTAAAGCATAGTCAC10200.12181140728002293No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10150.12121429253845419No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG10100.12061717779688545No Hit
CTATTCAGACTCCGTGAGGGGCCGATTCACCGTCTCCAGGGACAATTCCA9820.1172733352441005No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9660.11536256807108053No Hit
ATACACAGCCGTGTCTTCTGGTCTCAGGCTGCTCATTTGCAGATATAGGG9470.11309353205311931No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT9440.11273526320817809No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9300.1110633419317856No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG9290.11094391898347188No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9210.10998853539696189No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9200.10986911244864814No Hit
CTTTTAGGAGGTGTCCGGTGTCAAGTGGACCTGGTGGAGTCGGGGGGAGG9170.10951084360370689No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9160.10939142065539313No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC8980.10724180758574567No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8840.10556988630935321No Hit
GTCTGAATAGTATTTAGTAGCTTCAAGATATGATATAGTTGCCACCCACT8710.10401738798127447No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8620.10294258144645076No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA8560.10222604375656826No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8540.10198719785994076No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8530.10186777491162703No Hit
GCACTGCACACAGAGGACTCACCATGGAGTTTGGGCTGACCTGGGTTTTC8500.10150950606668578No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC8420.1005541224801758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15300.060.2565081
TCAACGC16400.055.7557564
ATCAACG16500.055.4244543
CAACGCA17250.053.008375
AACGCAG18150.050.7765436
TATCAAC18350.050.229122
CGCAGAG19850.046.4251488
GCAGAGT23300.039.242059
ACGCAGA27000.034.1310847
CAGCGCT4300.031.8118559
GAATACG2351.3606041E-930.6362789
TACATGG18650.029.7299082
ACATGGG18750.028.803263
GTACATG18850.028.6573031
GTACACG8400.028.2855365
GTATGTA10550.027.9908981
GTGCTAG3153.8198777E-1127.438231
TACACGG8700.027.3101776
GGTATCA15050.027.2787061
GCAGCGC5100.026.821768
Produced by FastQC (version 0.11.5)