FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208395
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGAAACA28400.23502248850748308No Hit
GTCTATTACTGTGCGAGAGATGAAAATTGTAGTGGTGCCAATTGCTATCC22470.1859491308719417No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC18500.15309563511931115No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCGCTG18300.15144054717207536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18110.14986821362220135No Hit
CCTATGTGCTGACTCAGCCTCCCTCAGTCTCAGTGGCCCCAGGGAAGACG15740.13025542144745716No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA15140.12529015760574977No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTCCCGAAGACATAATGATCACTCC14550.12040764816140417No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14510.12007663057195701No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14450.11958010418778628No Hit
CCCCTATCCTGGTCATCTGTTATGATTCCGACCGGCCCTCAGGGATCCCT14150.11709747226693258No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT14080.11651819148540006No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTCCCGAAGACATAATGATCACTC14080.11651819148540006No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGC13580.11238047161731056No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13230.10948406770964793No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12990.10749796217296498No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12870.1065049094046235No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12660.1047670670600259No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC12360.10228443513917221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22950.080.508011
TCAACGC26700.067.992494
ATCAACG27200.066.742623
CAACGCA27500.066.011795
AACGCAG29350.062.039826
TATCAAC29850.061.300082
GGTATCA9450.057.2810971
CGCAGAG34150.052.687488
ACGCAGA36400.050.023877
GCAGAGT41050.044.1820149
TAGACGG11800.042.733625
ATAGACG13100.038.4944734
AATAGAC15500.033.9283523
ACATGGG32700.032.1644973
TAATAGA17150.029.8242
GTACATG41600.028.106291
TACATGG41800.026.8856142
CAGAGTA38750.026.3739249
GAATCGT2301.1761349E-625.0334246
CTGTGCG14100.025.0112349
Produced by FastQC (version 0.11.5)