FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605139
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACCGCCGTGTCCTCGACTCTCAGGCTGTTCAGTTGTAGATATA20290.33529486613819304No Hit
GTGTATTACTGTGCGAGAGGGCTACCCGCCTTCGACACCTGGGGCCGTGG16740.27663065841071227No Hit
ATCTACAACTGAACAGCCTGAGAGTCGAGGACACGGCGGTGTATTACTGT13850.2288730357818617No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12740.21053014266143813No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12610.20838187589958668No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11690.19317875727725364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11070.18293317733611617No Hit
GTTGTAGATATAGTGATTTCTCGGCGCTGTCTCTGGAGATGGTGAATCGG10980.1814459157317575No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT10930.18061965928489157No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10390.17169608965873956No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9760.16128525842822888No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9760.16128525842822888No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9540.15764973006201882No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA9530.15748447877264563No Hit
GTAATAATCAGCCTCATCCTCAGCCCGGAGCCCAGAGATGGTCAGGGAGG9430.15583196587891376No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9250.15285744267019644No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9220.1523616888020769No Hit
TCTCTGGGCTCCGGGCTGAGGATGAGGCTGATTATTACTGCTGTTCATTT9060.1497176681721059No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT9020.14905666301461318No Hit
GATTATTACTGCTGTTCATTTACAAACAGCCCCACTTCCTTTTATGTCTT9020.14905666301461318No Hit
CTCCAGAGACAGCGCCGAGAAATCACTATATCTACAACTGAACAGCCTGA9020.14905666301461318No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8970.14823040656774725No Hit
ACTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGTCCCCAA8870.14657789367401539No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8620.14244661143968576No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8530.1409593498353271No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC8170.1350103034178924No Hit
ATATAAAGCACGATGGAAGTGAAAAATACTATGTGGACTCTGTGAAGGGC8160.13484505212851922No Hit
GTCTCTGGAGATGGTGAATCGGCCCTTCACAGAGTCCACATAGTATTTTT7930.13104427247293596No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC7640.1262519850811136No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC7530.12443422089800854No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT7420.12261645671490352No Hit
CCTTTAGTACCTATTGGATGACTTGGGTCCGCCAGGCTCCAGGGAAGGGG7380.12195545155741078No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT7370.1217902002680376No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7320.12096394382117166No Hit
ACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAAGGAAGTGG7160.1183199231912007No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7160.1183199231912007No Hit
GTATTACTGTGCGAGAGGGCTACCCGCCTTCGACACCTGGGGCCGTGGCA6960.115014897403737No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA6850.11319713322063196No Hit
TTTACAAACAGCCCCACTTCCTTTTATGTCTTCGGAACTGGGACCAAGGT6780.11204037419501967No Hit
GTGTCCGGGTCTCCTGGGCAGTCAGTCACCATCTCCTGCACTGGAACCAG6780.11204037419501967No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6690.11055311259066099No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG6620.10939635356504869No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC6380.10543032262009225No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG6370.10526507133071906No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6340.1047693174625995No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG6300.10410831230510675No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA6270.1036125584369872No Hit
CAGTAATACACCGCCGTGTCCTCGACTCTCAGGCTGTTCAGTTGTAGATA6260.10344730714761402No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG6250.10328205585824082No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTACTTATAACTATGTCTCCT6120.10113378909638943No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)