FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605139
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACCGCCGTGTCCTCGACTCTCAGGCTGTTCAGTTGTAGATATA20210.33397285582320757No Hit
GTGTATTACTGTGCGAGAGGGCTACCCGCCTTCGACACCTGGGGCCGTGG16210.2678723400739334No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15320.25316497531971993No Hit
ATCTACAACTGAACAGCCTGAGAGTCGAGGACACGGCGGTGTATTACTGT13990.23118655383308628No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11860.1959880291965978No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11690.19317875727725364No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10750.17764513607617424No Hit
GTTGTAGATATAGTGATTTCTCGGCGCTGTCTCTGGAGATGGTGAATCGG10630.17566212060369601No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT10590.17500111544620328No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10250.16938257160751496No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA10160.1678953100031563No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT9830.16244201745384118No Hit
GATTATTACTGCTGTTCATTTACAAACAGCCCCACTTCCTTTTATGTCTT9320.15401420169580873No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9250.15285744267019644No Hit
GTAATAATCAGCCTCATCCTCAGCCCGGAGCCCAGAGATGGTCAGGGAGG9200.1520311862233305No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9100.15037867332959864No Hit
TCTCTGGGCTCCGGGCTGAGGATGAGGCTGATTATTACTGCTGTTCATTT9090.15021342204022547No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8670.1432728678865517No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8650.14294236530780532No Hit
ACTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGTCCCCAA8510.1406288472565807No Hit
CTCCAGAGACAGCGCCGAGAAATCACTATATCTACAACTGAACAGCCTGA8320.1374890727584902No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8080.13352304181353375No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7990.13203578020917509No Hit
ATATAAAGCACGATGGAAGTGAAAAATACTATGTGGACTCTGTGAAGGGC7960.13154002634105552No Hit
GTCTCTGGAGATGGTGAATCGGCCCTTCACAGAGTCCACATAGTATTTTT7900.13054851860481642No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7880.13021801602607003No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC7550.12476472347675494No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7540.12459947218738175No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC7390.12212070284678396No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7380.12195545155741078No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC7240.11964193350618617No Hit
GTATTACTGTGCGAGAGGGCTACCCGCCTTCGACACCTGGGGCCGTGGCA7210.11914617963806663No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7110.11749366674433477No Hit
CCTTTAGTACCTATTGGATGACTTGGGTCCGCCAGGCTCCAGGGAAGGGG7040.11633690771872247No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA6960.115014897403737No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG6790.11220562548439285No Hit
ACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAAGGAAGTGG6780.11204037419501967No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG6780.11204037419501967No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT6610.1092311022756755No Hit
TTTACAAACAGCCCCACTTCCTTTTATGTCTTCGGAACTGGGACCAAGGT6550.1082395945394364No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT6540.1080743432500632No Hit
GTGTCCGGGTCTCCTGGGCAGTCAGTCACCATCTCCTGCACTGGAACCAG6520.10774384067131684No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6510.10757858938194366No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA6410.1059260764882118No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT6410.1059260764882118No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC6300.10410831230510675No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG6250.10328205585824082No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTACTTATAACTATGTCTCCT6160.10179479425388217No Hit
CAGTAATACACCGCCGTGTCCTCGACTCTCAGGCTGTTCAGTTGTAGATA6150.10162954296450899No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)