FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675066
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC51340.30649538585345293No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT47510.2836306151518806No Hit
GTAATAATGAGCCTCGTCTTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG44700.2668551567520325No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC43880.26195982725456785No Hit
CTCCTGGACAGTCGCTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT39540.23605040040213343No Hit
CATTATTACTGCTGCTCATATGCAGGTGATAGTATTTATGTCTTCGGAAC38190.22799101647338074No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT37260.22243899643357334No Hit
TTATTACTGCTGCTCATATGCAGGTGATAGTATTTATGTCTTCGGAACAG35680.21300653227992208No Hit
ACCTAGGACGGTGACCCTGGTCCCTGTTCCGAAGACATAAATACTATCAC34610.20661872427713296No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA34600.20655902513691998No Hit
GACCTAGGACGGTGACCCTGGTCCCTGTTCCGAAGACATAAATACTATCA34590.20649932599670698No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC33110.19766385324518557No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32530.19420130311283257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32130.19181333750431326No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC31020.18518673294067217No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA30370.1813062888268283No Hit
GTATTTATGTCTTCGGAACAGGGACCAGGGTCACCGTCCTAGGTCAGCCC29890.17844073009660516No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT29310.17497817996425216No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAACCTTGTCTCCT29300.17491848082403916No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC29030.17330660403828863No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG28630.17091863842976934No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27270.1627995553608037No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG27200.1623816613793128No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC26960.16094888201420124No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26640.1590385095273858No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA25330.15121792215948504No Hit
CATAAATACTATCACCTGCATATGAGCAGCAGTAATAATGAGCCTCGTCT25100.14984484193458644No Hit
GCTCATATGCAGGTGATAGTATTTATGTCTTCGGAACAGGGACCAGGGTC24450.14596439782074258No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT22700.1355170482834706No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA21970.13115901104792288No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG20160.12035346666937302No Hit
GGTGATAGTATTTATGTCTTCGGAACAGGGACCAGGGTCACCGTCCTAGG19890.11874158988362249No Hit
CATATGAGCAGCAGTAATAATGAGCCTCGTCTTCAGCCTGGAGCCCAGAG19550.1167118191163811No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG19250.1149208449099916No Hit
GCAGTAATAATGAGCCTCGTCTTCAGCCTGGAGCCCAGAGATTGTCAGGG19090.1139656586665839No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCT18650.11133889649721264No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC18470.11026431197337896No Hit
TTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAAC18380.1097270197114621No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG17790.10620477043889615No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC17700.10566747817697929No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17660.10542868161612737No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17120.10220492804462629No Hit
GCATATGAGCAGCAGTAATAATGAGCCTCGTCTTCAGCCTGGAGCCCAGA17080.10196613148377438No Hit
GCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCTGTTCCGAAGACATA17070.10190643234356139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.10130944094143156No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16250.079.788051
GTATCAA42900.067.497471
TCAACGC46550.060.941584
ATCAACG46900.060.7938353
TATCAAC48950.058.5422
CAACGCA49700.057.2239575
AACGCAG52350.054.4647676
ACGCAGA56600.050.246397
CGCAGAG58600.048.6543668
GCAGAGT71550.039.84839
GAATACG5800.037.2406659
TATAACG950.00489071130.3161183
GTGCTAG8250.029.6854531
GTACATG50000.028.3801671
CTATACC6900.027.1307284
TACATGG50750.026.6722092
ACATGGG51200.026.0159053
TCTACAC16200.025.7780573
CAGAGTA67600.025.4551379
CCTATAC5950.025.4120373
Produced by FastQC (version 0.11.5)