FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675066
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC43150.2576017900190201No Hit
GTAATAATGAGCCTCGTCTTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG41920.2502587957728233No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT40350.24088603075938503No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC37270.22249869557378635No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT37220.2222001998727214No Hit
CATTATTACTGCTGCTCATATGCAGGTGATAGTATTTATGTCTTCGGAAC36990.2208271196478228No Hit
CTCCTGGACAGTCGCTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT34990.2088872916052263No Hit
TTATTACTGCTGCTCATATGCAGGTGATAGTATTTATGTCTTCGGAACAG32750.1955146841975182No Hit
GACCTAGGACGGTGACCCTGGTCCCTGTTCCGAAGACATAAATACTATCA31820.1899626641577108No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31360.1872165037079136No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31060.1854255295015241No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA30940.18470913981896833No Hit
ACCTAGGACGGTGACCCTGGTCCCTGTTCCGAAGACATAAATACTATCAC30880.18435094497769042No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC28880.1724111169350939No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA28030.1673366900169904No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC27210.1624413605195258No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC26320.15712813704057033No Hit
GTATTTATGTCTTCGGAACAGGGACCAGGGTCACCGTCCTAGGTCAGCCC25700.15342679034736542No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25430.1518149135616149No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAACCTTGTCTCCT24880.14853146084990082No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC24650.14715838062500222No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT24050.14357643221222327No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA23870.1425018476883896No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG23840.14232275026775065No Hit
CATAAATACTATCACCTGCATATGAGCAGCAGTAATAATGAGCCTCGTCT23440.13993478465923134No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG22970.13712892506922114No Hit
GCTCATATGCAGGTGATAGTATTTATGTCTTCGGAACAGGGACCAGGGTC22070.13175600245005273No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21830.13032322308494113No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT20480.12226383915618848No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA20190.12053256409001198No Hit
CATATGAGCAGCAGTAATAATGAGCCTCGTCTTCAGCCTGGAGCCCAGAG18830.11241348102104633No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG18570.1108613033755088No Hit
GCAGTAATAATGAGCCTCGTCTTCAGCCTGGAGCCCAGAGATTGTCAGGG18080.10793604550507264No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC17740.10590627473783122No Hit
GGTGATAGTATTTATGTCTTCGGAACAGGGACCAGGGTCACCGTCCTAGG17420.10399590225101578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17300.10327951256845999No Hit
TTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAAC16970.10130944094143156No Hit
ACTATCACCTGCATATGAGCAGCAGTAATAATGAGCCTCGTCTTCAGCCT16850.10059305125887576No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)