FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411998
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC41670.2951137324557117No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGATGATCACTA39180.2774791465710291No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG33560.2376773904778902No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGG32790.2322241249633498No Hit
CTACTAGAATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC28550.20219575381834817No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28190.19964617513622543No Hit
TTCTAGTAGTGATCATCCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC26980.1910767578990905No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA23610.16720986856921893No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23320.16515604129750894No Hit
CCCTTAGTGTCGGTGGCCCCAGGAAAGCCGGCCAGGATTACCTGTGGGGG22590.1599860623032044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21700.15368293722795642No Hit
CCTATGTGCTGACTCAGCCACCCTTAGTGTCGGTGGCCCCAGGAAAGCCG21280.15070842876547982No Hit
ATGTACTACTGTGCGAAACAGGTCGATAGCAGTGGAACGGAGGCCTTTGA21270.15063760713542088No Hit
GTAGTACATGGCGGTGTCCGAGGCCCTCAGGCTGGTCCACTGCAGGTAGA20950.14837131497353395No Hit
GTGTTGGTCATCTATTATGATAATGACCGGCCCTCAGGGATCCCTGAGCG19610.13888121654563249No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT19340.1369690325340404No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGATGATCACTAC19160.135694243192979No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC18950.13420698896174074No Hit
GTATAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGTTG18720.13257809147038452No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18180.12875372344720035No Hit
GCCCTCAGGGATCCCTGAGCGGTTCTCTGGCTCCAACTCTGGAAACATGG17990.12740811247608No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17500.12393785260319065No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17250.1221673118517165No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA17170.12160073881124478No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA17140.12138827392106787No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA16260.11515597047587886No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC15850.11225228364346125No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14980.10609080182833121No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14960.10594915856821327No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT14750.10446190433697497No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14680.10396615292656221No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC14680.10396615292656221No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTATACTT14490.10262054195544186No Hit
CCCCTGTGTTGGTCATCTATTATGATAATGACCGGCCCTCAGGGATCCCT14430.10219561217508807No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14400.10198314728491117No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTTAGTGTCGGTGGC14400.10198314728491117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24700.076.415121
TCAACGC30350.061.9236874
ATCAACG30750.060.8840073
CAACGCA32100.058.770035
AACGCAG33450.056.394156
TATCAAC35250.053.7244072
CGCAGAG39750.047.4562038
ACGCAGA41050.045.953337
GCAGAGT46950.040.3319329
GGTATCA19750.039.3941421
ACATGGG40300.036.271543
GTACATG50100.032.7847671
CATGGGA19550.031.3074154
TACATGG51300.030.8802152
TATTGAG16700.028.0257035
CAGAGTA44400.027.5673399
GTAGTAC15800.025.9891911
TAGTACA16200.025.3358142
TGGCGGT15350.024.8597269
TTATTGA19750.024.4277084
Produced by FastQC (version 0.11.5)