FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921919
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTACTGTGCGAGGGTTGATTGTAGTGGTACCAGCTGCTATCAGCACT24060.26097737436803015No Hit
GTAATATAGGGCCGAGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGG22270.2415613519192033No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21950.23809033114622868No Hit
CTATATTACTGTGCGAGGGTTGATTGTAGTGGTACCAGCTGCTATCAGCA20910.22680951363406116No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT18680.20262083762239416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18460.20023451084097407No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC16450.1784321616107272No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA16080.17441879384197526No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15700.1702969566740679No Hit
GCCCTATATTACTGTGCGAGGGTTGATTGTAGTGGTACCAGCTGCTATCA14810.16064318014923218No Hit
ATATAGGGCCGAGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGGCTG14780.16031777195176583No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13200.14317960688520356No Hit
GCCTACATGGAACTGAGCAGCCTGAGATCTGAGGACTCGGCCCTATATTA12820.1390577697172962No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12220.13254960576796876No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11520.12495674782708677No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11100.12040103306255755No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10950.1187739920752257No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10600.1149775631047847No Hit
CAGTAATATAGGGCCGAGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTA10110.10966256254616728No Hit
ACATGGAACTGAGCAGCCTGAGATCTGAGGACTCGGCCCTATATTACTGT9990.1083609297563018No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC9880.10716776636559178No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9830.10662541936981447No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT9780.10608307237403719No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA9690.10510684778163809No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9670.10488990898332717No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9540.10347980679430621No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9530.10337133739515077No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9510.10315439859683985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)