FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921919
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTACTGTGCGAGGGTTGATTGTAGTGGTACCAGCTGCTATCAGCACT24810.26911257930468946No Hit
GTAATATAGGGCCGAGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGG23860.25880798638492103No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19810.21487787972696085No Hit
CTATATTACTGTGCGAGGGTTGATTGTAGTGGTACCAGCTGCTATCAGCA19480.21129838955483074No Hit
ATATAGGGCCGAGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGGCTG16200.17572042663184076No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT16080.17441879384197526No Hit
GCCCTATATTACTGTGCGAGGGTTGATTGTAGTGGTACCAGCTGCTATCA15760.17094777306900064No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14180.1538096080024384No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA14160.15359266920412748No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC13490.14632521946071184No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12650.13721378993165342No Hit
GCCTACATGGAACTGAGCAGCCTGAGATCTGAGGACTCGGCCCTATATTA11930.12940399319246051No Hit
CAGTAATATAGGGCCGAGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTA11350.12311276804144398No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10970.11899093087353661No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10320.11194041992843189No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9510.10315439859683985No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9380.10174429640781892No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9260.10044266361795343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC12450.065.275784
ATCAACG12950.062.748663
GTATCAA13150.062.4191441
CAACGCA13000.061.9642565
AACGCAG14650.054.9972956
TATCAAC15250.052.3418352
GGTATCA5400.049.334041
CGCAGAG16750.048.104738
ACGCAGA16900.047.6751757
TGTAGCG700.001099854441.0920452
GCAGAGT21000.038.3692479
GAATACG3500.036.9989179
GTACATG24150.029.217811
GACCGCG1358.45773E-426.6438647
TCTACAC9550.025.6018473
GCTAGAG6000.025.1688773
GTGCTAG4900.023.5105441
TAGGGCC15950.023.4532817
TATAGGG17200.022.998575
ATAATAC4700.022.9503423
Produced by FastQC (version 0.11.5)