FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821492
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTAGTAGCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGACTTC15100.1838118934816164No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG14320.17431697448057926No Hit
GCCTTGGGCTGACCGAGGACGGTCAGGTGGGTGCCTCCTCCGAACACAGC13710.16689146090284507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12570.15301427159363695No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG11990.14595394720825033No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11900.1448583796312076No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC10430.12696410920617607No Hit
CCTTTAGTTCCAATGTTGTCACCCCCACAGGTCATCCTGGCCGTCTTTCC10360.12611200109069837No Hit
TCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGACCGATTCTCTGGC9360.11393902801244565No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGC9170.11162616312757763No Hit
GTAATAGTCGGCCTCATCCCCGACTTCGACCCTGGCGATGGTCAGGGTGG9130.11113924420444751No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA9030.10992194689662224No Hit
CTAGCAAACTGATCACTACTAGTAGCCCACACCTGACAGTAATAGTCGGC9010.10967848743505719No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG8860.10785254147331928No Hit
ACACAGGGGCCTGACCTGGCTGCTGCAGGTACCAGTGCACACCTTTAGTT8700.10590486578079883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24900.048.611
GTTACGT450.00990159148.004993
TCAACGC27700.042.6274034
ATCAACG27600.042.526163
CAACGCA28300.041.723645
TATCAAC29750.040.178972
AACGCAG30050.039.533416
CGCAGAG30400.039.0758748
ACGCAGA30650.038.7571457
TATAACG750.00153375338.403992
GCAGAGT35750.033.630929
TGTTACG950.00488704830.3189432
ACACCGT1753.9829756E-628.7994886
TACATGG31950.026.8196892
CATGGGG14250.026.7784734
GTACATG31600.026.2196241
ACATGGG32750.025.06523
GGTATCA17000.023.309311
GTATATG5050.022.8267611
GTCTATA5450.022.4733641
Produced by FastQC (version 0.11.5)