FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614391
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8940.1455099439933202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12000.070.2192461
TCAACGC12700.064.059694
CAACGCA12900.063.0665255
ATCAACG13000.062.5813943
AACGCAG13300.061.169786
TATCAAC14250.057.09182
CGCAGAG16150.050.8209158
ACGCAGA17950.045.3235747
GCAGAGT19950.041.862519
GGTATCA7550.031.4788251
GTCTAGA2156.483733E-726.7980461
TTAGTAC1801.5929299E-423.9987684
GTACATG14800.023.8443741
CAGAGTA18750.023.4227969
GTATATA4609.094947E-1221.9190521
GTCCTAG3004.8494985E-721.6059231
TACATGG15250.021.2448082
GTGTATA3458.0375685E-820.8752881
TACACTG5201.8189894E-1220.7681625
GTATAGG2908.728082E-619.8675161
Produced by FastQC (version 0.11.5)