FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614391
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC6450.058.0550464
GTATCAA6600.057.8455281
ATCAACG6700.054.814033
CAACGCA7000.053.489225
AACGCAG7500.049.9151466
TATCAAC8150.047.7203062
CGCAGAG8850.042.3044178
TATACCG700.001092550941.1455575
ACGCAGA10800.034.6632967
ACTACGC3101.8189894E-1230.193078
GCAGAGT12450.030.0718149
TACCGTA1204.2264693E-429.9970847
GTACATG13750.029.3375071
GTAATAC2405.6221324E-827.0039713
GGTATCA5900.026.8601551
TCTATAC2955.2204996E-1026.8514063
TACATGG13450.026.7740962
CTACGCA3555.456968E-1226.3657829
ACATGGG14250.024.7615363
TACTACG4002.910383E-1123.3977247
Produced by FastQC (version 0.11.5)