FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657078
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACG19460.29615966445383957No Hit
GCTATGCACTGGGTCCGCCAGGCTTCCGGGAAAGGGCTGGAGTGGGTTGG19170.2917461853843836No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18710.28474549444662584No Hit
GAATACAGGAGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT17670.2689178453699561No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14880.22645713294312092No Hit
TCTCTGGAGATGGTGAACCTGCCTTTCACCGACGCACCATATGCTGTCGC13390.20378098186212293No Hit
CCATATGCTGTCGCGTAACTGTTAGCTTTGGTTCTAATATGGCCAACCCA12470.18977959998660737No Hit
GTAGTACAGTAATACACGGCCGTGTCCTCGGTTTTCAGGCTGTTCATTTG12440.18932303318631882No Hit
GCATATGGTGCGTCGGTGAAAGGCAGGTTCACCATCTCCAGAGATGATTC11570.17608259597795087No Hit
ATACACGGCCGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACGCCG11330.17243006157564245No Hit
TATTAGAACCAAAGCTAACAGTTACGCGACAGCATATGGTGCGTCGGTGA11250.17121255010820632No Hit
GTATTACTGTACTACCGGCCCTGCGTCATCAGATGCTTTTGATATCTGGG11030.16786439357275698No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11010.16756001570589793No Hit
CTCCAGAGATGATTCAAAGAGCACGGCGTATCTGCAAATGAACAGCCTGA10960.16679907103875036No Hit
GTGTATTACTGTACTACCGGCCCTGCGTCATCAGATGCTTTTGATATCTG10710.1629943477030124No Hit
GTGCATAGCAGAGCCACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTT10590.16116808050185824No Hit
CAGTAATACACGGCCGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATA10480.15949400223413354No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10420.15858086863355644No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG10130.15416738956410048No Hit
TTACTGTACTACCGGCCCTGCGTCATCAGATGCTTTTGATATCTGGGGCC9970.15173236662922818No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT9960.15158017769579868No Hit
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTC9890.15051485516179205No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9530.14503605355832944No Hit
CTGTACTACCGGCCCTGCGTCATCAGATGCTTTTGATATCTGGGGCCAAG9460.14397073102432284No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGG9050.13773098475371265No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG8630.13133904954967293No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8580.13057810488252536No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC8550.1301215380822368No Hit
GTACAGTAATACACGGCCGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAG8490.1292084044816597No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8340.12692557048021696No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT8290.1261646258130694No Hit
GCCGTGTATTACTGTACTACCGGCCCTGCGTCATCAGATGCTTTTGATAT8100.12327303607790857No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC8010.12190333567704292No Hit
GTACATGGGAGCTCTGGGAGAGGAGCTCCAGCCTTGGGATTCCCAGCTGT7860.11962050167560016No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC7530.11459826687242611No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC7480.11383732220527852No Hit
ATACAGGAGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGTTG7440.11322856647156046No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT7290.11094573247011771No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7130.10851070953524543No Hit
AATATGGCCAACCCACTCCAGCCCTTTCCCGGAAGCCTGGCGGACCCAGT6990.1063800644672322No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6720.10227096326463525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11550.076.09551
GGTATCA4700.062.844481
TCAACGC13400.062.3260844
ATCAACG13550.061.6408233
CAACGCA13650.061.1845865
TATCAAC13850.060.305642
AACGCAG14350.058.199976
GTCGTAA450.00988240748.027491
CGCAGAG17450.047.860728
GCAGAGT18600.045.6757559
ACGCAGA19700.042.7598657
TAGGTGG6950.041.437345
GTCATAG7200.040.0229071
GTGCTAG1452.0370862E-839.7468871
ATAGGTG6800.039.175044
CAGAGCG7000.039.0842939
ACAGAGC8000.036.8986558
CATGGGA7400.034.0528144
GTCTTAG1102.527697E-432.7460141
GAATACG1102.5367498E-432.7260789
Produced by FastQC (version 0.11.5)