FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080298
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTACACTATCCCTTCGTACACCTTTGGCCAGGGGACCAAGCTCGAGA47910.44348874106959374No Hit
GTGTAAGCCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCACACC43570.4033146409601795No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34410.31852322229607016No Hit
GTAATATACAGCCGTGTCTTCGGCTCTCAGGTTGTCCATTTCAAGATAGA26790.24798712947723683No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25210.2333615354281874No Hit
CCATTGATGGTGAGATTGAAAATTGTCCCAGACTCACTGGCGCTGAACCT18810.17411862282444288No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18270.16912000207350195No Hit
GTATATTACTGCGCGACGGCGACTGATAGACAAGTACTCCCAGTTGTGAA17190.15912276057162006No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16460.15236536585275542No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA15510.14357149601313712No Hit
ATATACAGCCGTGTCTTCGGCTCTCAGGTTGTCCATTTCAAGATAGAGTG15170.1404242162810632No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15030.13912827756785628No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14920.13811004000747942No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14910.13801747295653607No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14860.13755463770181933No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14740.1364438330904991No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13790.12764996325088077No Hit
CCTTTGGCCAGGGGACCAAGCTCGAGATCAAACGAACTGTGGCTGCACCA13190.12209594019427972No Hit
TCTAAAAGGTGTCTACTGTGAGGTGCACCTAGTGGAATCTGGGGGAGCCC13130.12154053788861963No Hit
GATCTATGCTGCGTCCCGTTTGCACAGTGGCGCCCCATCAAGGTTCAGCG12880.11922636161503584No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12870.1191337945640925No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12850.1189486604622058No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12700.11756015469805553No Hit
CTCCTGATCTATGCTGCGTCCCGTTTGCACAGTGGCGCCCCATCAAGGTT12000.11108046113202097No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11880.10996965652070077No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGA11760.10885885190938056No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11390.10543387102447657No Hit
GTGGCGCCCCATCAAGGTTCAGCGCCAGTGAGTCTGGGACAATTTTCAAT11340.10497103576975983No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA11260.10423049936221303No Hit
CACTATCCCTTCGTACACCTTTGGCCAGGGGACCAAGCTCGAGATCAAAC10890.10080551847730904No Hit
GTGGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGAGCATTAGCAG10840.1003426832225923No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)