FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080298
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTACACTATCCCTTCGTACACCTTTGGCCAGGGGACCAAGCTCGAGA45310.4194213078243226No Hit
GTGTAAGCCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCACACC41900.38785594345263996No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34790.32204077023191746No Hit
GTAATATACAGCCGTGTCTTCGGCTCTCAGGTTGTCCATTTCAAGATAGA26990.24983847049610386No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24440.22623387250554938No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17590.16282544260935408No Hit
CCATTGATGGTGAGATTGAAAATTGTCCCAGACTCACTGGCGCTGAACCT17160.15884505941878999No Hit
GTATATTACTGCGCGACGGCGACTGATAGACAAGTACTCCCAGTTGTGAA16250.15042145778294508No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15370.1422755572999302No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15250.14116475268860998No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA15240.14107218563766663No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14970.13857287526219617No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14550.13468505912257545No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14300.13237088284899168No Hit
ATATACAGCCGTGTCTTCGGCTCTCAGGTTGTCCATTTCAAGATAGAGTG14100.13051954183012465No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13340.12348444595842997No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12670.11728245354522548No Hit
CCTTTGGCCAGGGGACCAAGCTCGAGATCAAACGAACTGTGGCTGCACCA12280.1136723385584348No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12220.11311693625277469No Hit
GATCTATGCTGCGTCCCGTTTGCACAGTGGCGCCCCATCAAGGTTCAGCG12130.11228383279428453No Hit
TCTAAAAGGTGTCTACTGTGAGGTGCACCTAGTGGAATCTGGGGGAGCCC11690.1082108825527771No Hit
GTGGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGAGCATTAGCAG11510.10654467563579678No Hit
CTCCTGATCTATGCTGCGTCCCGTTTGCACAGTGGCGCCCCATCAAGGTT11430.10580413922825No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11170.10339739590372286No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11090.10265685949617605No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGA11030.10210145719051596No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10930.10117578668108243No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12000.069.1020051
TCAACGC13850.057.7678344
ATCAACG14200.056.3439753
TATCAAC16750.048.1965682
CGCAGAG18000.044.4264688
CAACGCA17900.044.3050545
AACGCAG18900.042.3089566
AATCCGC3150.041.165476
AAATCCG3300.039.3253145
TCCGCTA3600.036.0214588
GCAGAGT23550.033.9565359
ACATGGG26600.033.330083
TACATGG26750.032.8737222
GTACATG27000.032.8484951
TACACGG6750.032.017596
GTACACG6800.031.807245
CCGCTAC4100.031.62869
GTAAGCC23000.030.7122143
GTAATAT16450.030.6835941
ATCCGCT4250.030.5122957
Produced by FastQC (version 0.11.5)