FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022030
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCATCGTCGGACCGGAGCCCACTGATGGCCAGGGAGG48690.4764048022073716No Hit
GACTATTACTGTGCATCATGGGATGACAGCCTGAGCGGTTGGGTGTTCGG38290.37464653679441895No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC29620.28981536745496705No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACCGCTCAGG23250.22748842988953355No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGTGTCT18790.18384978914513272No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18330.17934894279032906No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT18230.17837049793058912No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGTGTCTGGGACCC17370.16995587213682573No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCA17090.16721622652955392No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC17010.16643347064176198No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16900.16535718129604807No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16570.1621283132589063No Hit
CTATTACTGTGCATCATGGGATGACAGCCTGAGCGGTTGGGTGTTCGGCG16150.1580188448479986No Hit
CTATAGGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCT15700.15361584297916892No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC15240.14911499662436523No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14540.14226588260618572No Hit
CAGTAATAGTCAGCCTCATCGTCGGACCGGAGCCCACTGATGGCCAGGGA14440.1412874377464458No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGCTCAGCTCCATGTAGG14390.14079821531657585No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14340.14030899288670587No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14320.14011330391475788No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14110.13805856970930402No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT13660.13365556784047436No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA13520.13228574503683846No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13360.13072023326125456No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA13000.12719783176619082No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA12690.12416465270099702No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12660.12387111924307506No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12650.12377327475710106No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACCGCTCAGGC12640.12367543027112707No Hit
GTGCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGCACAGGATCAC12460.1219142295235952No Hit
TTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA12420.12152285157969922No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT12210.11946811737424537No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT12200.11937027288827137No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCCTCTTGGTGGGAGCAG11540.11291253681398786No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG11500.11252115887009188No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11480.11232546989814389No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10950.10713971214152226No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10880.1064548007397043No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10720.10488928896412043No Hit
GCCTACATGGAGCTGAGCAGCCTGAGAGCCGAGGACACGGCCGTGTATTA10520.10293239924464057No Hit
GTGTATTACTGTGCGGCAGCTGATAGTTTTGGAGTGGTCATTGGGGTGAA10400.10175826541295266No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA10300.10077982055321272No Hit
GTCTGGGACCCCCGGGCACAGGATCACCATCTCTTGTTCTGGAAGCAGCT10270.10048628709529075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8850.079.051161
GTATCAA22350.078.0939941
ATCAACG27300.063.601313
TCAACGC27950.062.1222154
CAACGCA28900.059.830845
TATCAAC29800.058.7491652
AACGCAG30900.056.144716
CGCAGAG35150.049.1466268
ACGCAGA36100.047.8532947
GCAGAGT38750.044.5807469
GTGCTAG3350.032.294331
CAGCGCT4700.030.6295019
TAACGCA950.004874734730.3352854
GTACATG32800.030.1249641
GTACACG6800.029.6661225
TATTGAG16300.028.730125
ACATGGG33350.028.3000453
CAGAGTA39250.027.8747969
TTATTGA17500.027.1717454
AGGCCGT2508.389361E-825.9150966
Produced by FastQC (version 0.11.5)