FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130113
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTGATCATTTGCAGAGACA22370.19794480728918257No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20910.18502574521308932No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17380.15378993074143915No Hit
ATTTATTACTGTGCGAAAGATTTGGGATACTGTACTAGTACCTTCTGCTC13540.11981102774678284No Hit
GTACTAGTACAGTATCCCAAATCTTTCGCACAGTAATAAATGGCCGTGTC13060.11556366487245082No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12910.11423636397422206No Hit
GTACTAGTACCTTCTGCTCTGAGACTTTTGACTCCTGGGGCCAGGGAACC12050.10662650549104383No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11770.10414887714768346No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13600.058.855334
ATCAACG14100.056.7682653
CAACGCA14950.053.5358855
GTATCAA15150.052.8572771
AACGCAG15950.050.126036
TATCAAC17300.045.850952
CGCAGAG19150.041.7498788
GTATACG850.002809290833.949671
GCAGAGT23800.033.8955049
GTATATA6750.032.0635761
GTACATG27800.030.362361
CAGAGTA22450.029.5170179
TACATGG28300.028.5386432
ACGCAGA29150.027.427457
GAATACG3306.730261E-1126.1919789
ACATGGG32950.023.4169353
ATGGCCG14650.022.1246248
GTTCTAT4256.548362E-1122.0672841
ACCGACC7900.021.8819088
GTATTAA6100.021.2881091
Produced by FastQC (version 0.11.5)