FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391428
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT53870.38715621649125936No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39650.2849590492644966No Hit
CACCATAAGCAATCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC31260.22466128322845305No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29200.20985634901698114No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28930.20791589647470082No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG28100.20195080162250578No Hit
GAGTTACAGTACCCCTCGGGTCACCTTCGGCCAAGGGACACGACTGGACC27660.19878858266471566No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGTTCCACTGCAGGTAGG27130.19497954619283211No Hit
ATGTATTACTGTGCGAGACATAGGTCATTTGCGGCAGAGATCGTCAACCC26720.19203293307307315No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGCATTGGGAG26690.19181732723504197No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG24300.17464072880522744No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24140.17349083100239468No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23510.16896310840374063No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22140.159117108466985No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21940.15767973621344403No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21610.15530807199510144No Hit
GATATAGCCCGTCCTTCCAAGGCCATGTCACCATCTCAGTCGACAAGTCT21180.15221772164998837No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT20990.15085221800912443No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGATT20040.14402469980480487No Hit
CGACTGGACCAAGGGACACGACTGGATATTAAACGAACTGTGGCTGCACC19080.1371253129878082No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18890.1357598093469443No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT18350.1318789042623837No Hit
GTCCAGTCGTGTCCCTTGGCCGAAGGTGACCCGAGGGGTACTGTAACTCT17750.12756678750176076No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA17570.1262731524735739No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCAGCTGATACCAATT17260.12404522548058541No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTCGGGTCACCTTCGGCCA17020.12232037877633625No Hit
GCATTGGGAGTTATTTAAATTGGTATCAGCTGAAACCAGGGAAAGCCCCT16790.12066740068476414No Hit
GTCTGGAGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAAGATCTCCTGTG16340.11743331311429696No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16000.11498978028327733No Hit
GTGCAGCCACAGTTCGTTTAATATCCAGTCGTGTCCCTTGGTCCAGTCGT15930.11448669999453798No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15760.11326493357902816No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGATTGCTTATGGTGAGAGTGA15760.11326493357902816No Hit
GATTTCACTCTCACCATAAGCAATCTGCAACCTGAAGATTTTGCAACTTA15680.11268998467761178No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15510.11146821826210196No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAG15500.11139634964942491No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA15340.11024645184659213No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15270.1097433715578528No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA15240.10952776571982166No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15050.10816226207895772No Hit
GTATTACTGTGCGAGACATAGGTCATTTGCGGCAGAGATCGTCAACCCTT14950.10744357595218726No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG14950.10744357595218726No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14940.1073717073395102No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14660.10535938618455286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC28600.050.846834
GTATCAA30400.050.807541
ATCAACG29150.049.8874553
CAACGCA29350.049.547515
AACGCAG30650.047.445986
TATCAAC31650.045.7638362
ACGCAGA33600.043.2803347
GAATACG4000.043.194639
CGCAGAG34500.042.1512838
GCAGAGT40900.035.5554859
GTGCTAG6350.032.96191
GTACATG31800.029.9595181
GGACCGT1555.772454E-527.8675046
CTAGAGA8450.025.5589544
ACATTAG11500.023.7883477
GACTCGG9450.023.6161127
TAGCCCG16250.023.4801585
GCTAGAG8600.023.4389463
ACATGGG40200.023.280693
CAGAGTA44150.022.991489
Produced by FastQC (version 0.11.5)