FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391428
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT46180.3318892533426092No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32010.2300514291792317No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGTTCCACTGCAGGTAGG24250.1742813857418422No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24060.17291588210097827No Hit
GAGTTACAGTACCCCTCGGGTCACCTTCGGCCAAGGGACACGACTGGACC24050.17284401348830122No Hit
CACCATAAGCAATCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC23920.1719097215234996No Hit
ATGTATTACTGTGCGAGACATAGGTCATTTGCGGCAGAGATCGTCAACCC23400.1681725536642931No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG23190.16666331279807506No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22500.16170437852335875No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGCATTGGGAG21800.15667357563596537No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21240.15264893332605065No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20150.14481525454425237No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19900.14301853922732616No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT19870.14280293338929503No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17650.1268481013749903No Hit
GATATAGCCCGTCCTTCCAAGGCCATGTCACCATCTCAGTCGACAAGTCT17270.12411709409326245No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGATT16980.12203290432562805No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16580.11915815981854612No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT16520.11872694814248383No Hit
GTCCAGTCGTGTCCCTTGGCCGAAGGTGACCCGAGGGGTACTGTAACTCT16440.11815199924106745No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG16400.11786452479035925No Hit
GTCTGGAGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAAGATCTCCTGTG16220.11657088976217238No Hit
CGACTGGACCAAGGGACACGACTGGATATTAAACGAACTGTGGCTGCACC15700.11283372190296587No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15450.11103700658603967No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA14530.10442509421975121No Hit
GTATTACTGTGCGAGACATAGGTCATTTGCGGCAGAGATCGTCAACCCTT14470.10399388254368892No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCAGCTGATACCAATT14470.10399388254368892No Hit
GCATTGGGAGTTATTTAAATTGGTATCAGCTGAAACCAGGGAAAGCCCCT14400.10349080225494958No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA14330.10298772196621025No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)