FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225591
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTTCGGCTGTCAGGCTGTTCATTTGCAGATACA35690.29120644652253486No Hit
GTGTATTACTGTGCGAGAGAGGGCATGTCTAACGGTCTTTGGAGTGGGCC19930.16261542390569123No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14290.11659680921286139No Hit
TCCAGAGACAGCGCCAACAACTCACTGTATCTGCAAATGAACAGCCTGAC12650.10321550990501724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC26500.054.332734
GTATCAA27950.054.244651
CAACGCA28400.050.9512755
ATCAACG28650.050.2553983
AACGCAG30050.047.914056
TATCAAC32050.045.8768542
CGCAGAG32300.044.3535048
GTAATAC19550.039.1452031
TATACGG750.001526881138.440612
CAGCCGT18800.037.910089
GCAGAGT41100.034.8568889
AATACAC22450.033.3498883
ACGCAGA46250.031.2868427
TACACAG32450.031.281085
GTACCGT950.004893498530.3119416
TAATACA26050.029.3284852
CCGACCG4750.028.7963459
GGTATCA16750.027.5857371
ACACAGC32600.025.837216
TGCACCG5900.025.6238655
Produced by FastQC (version 0.11.5)