FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938925
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCGACTGTCAGGCTGTTCATTTGTAGATATA14820.15784008307372793No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12460.1327049551348617No Hit
CTTATATAGTGATGGAAGCACCTGCTTGAGTTGGCTTCAGCAGAGGCCAG12010.12791224006177276No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11420.1216284580770562No Hit
GGTACACACTGGCCGTTCACTTTTGGCCAGGGGACCAAACTGGAGATCAA11010.11726176212157521No Hit
GAATATCAGTAGGGTGGAGGCTGAGGATGTTGGCGTTTACTACTGCATGC10580.11268205660729025No Hit
GTGTGTACCTTGCATGCAGTAGTAAACGCCAACATCCTCAGCCTCCACCC10220.10884788454881913No Hit
GTCTATTACTGTGCGAAGAGTGATCCAGAGCCAGACTGGTGGAGGGGTAC9880.10522672204915196No Hit
GTTAAAAGGTGTCCAGTGTGGGGTTCAATTGTTGGAGTCTGGGGGAGGCT9450.100647016534867No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23950.055.123151
ATCAACG27150.047.2018363
TCAACGC27350.046.5934264
CAACGCA27850.046.0154345
TATCAAC29150.044.7304422
AACGCAG28750.044.5749556
ACGCAGA30950.041.4064567
CGCAGAG31500.040.6834878
GCAGAGT36900.034.729819
GAATCGT950.00489119630.3141296
CGCTAAG1000.006222925628.856821
GGTATCA13850.028.1275861
CAGAGTA36350.024.5598689
TAGACGG8250.024.4350265
GTGCTAG3702.764864E-1023.397421
ACCGACC2802.505367E-723.141598
ATAGACG8400.023.141594
ACATGGG32500.023.038743
GTACATG35550.021.9165731
TAACGTG1650.002708212521.8169865
Produced by FastQC (version 0.11.5)