FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012638
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCAGGCTGCGCGCCCGAAAGGGTCAGGGCAAATTTGCCCCCACGGAG56170.27908645270535487No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46210.22959916288969998No Hit
GCCTGAGGATGAGGCTGAATATTACTGTTTGCTCTCTACTAATGCTTTTC43010.21369963202523254No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC42370.21051972585233908No Hit
GCCTTGGGCTGACCTAGGACGAACAGTTTGGTCCCTTCGCCGAACATGCG41580.20659452917017365No Hit
CTACTAATGCTTTTCGCATGTTCGGCGAAGGGACCAAACTGTTCGTCCTA40670.20207310008059073No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40520.20132780957131885No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT39330.195415171531095No Hit
TCCCTGGACACCTGCCCGCTTCTCAGCCTCCCTCCGTGGGGGCAAATTTG37780.1877138362686186No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37700.18731634799700692No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA35180.1747954674412388No Hit
CTCTCTGACTGTGGCCCCAGGAGGGTCAGTCACTCTCACCTGTGGCTCCA33230.16510669082070398No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT33170.16480857461699522No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32970.163814853937966No Hit
GTAATAGACGGCAGTGTCCTCGATTTGTAGGCCGTTCATTTGCAGAAACA32390.16093306396878126No Hit
ATATTACTGTTTGCTCTCTACTAATGCTTTTCGCATGTTCGGCGAAGGGA32230.1601380874255579No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA32020.15909468071257724No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA30430.15119460131429496No Hit
CTATTCTCTGATGTAAATGACAGGCGTCCCTGGACACCTGCCCGCTTCTC29720.14766689290374124No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28790.14304609174625543No Hit
GACCTAGGACGAACAGTTTGGTCCCTTCGCCGAACATGCGAAAAGCATTA28450.14135676659190574No Hit
GAATATTACTGTTTGCTCTCTACTAATGCTTTTCGCATGTTCGGCGAAGG27920.13872340679247833No Hit
GTAATATTCAGCCTCATCCTCAGGCTGCGCGCCCGAAAGGGTCAGGGCAA26970.13400323356708957No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC26620.13226422237878843No Hit
GTCTATTACTGTGCCACAGACGCCCCGAAGGACTGGGGCCGGGGAGCCCT26200.1301774089528271No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG25700.12769310725525404No Hit
CAGTAATATTCAGCCTCATCCTCAGGCTGCGCGCCCGAAAGGGTCAGGGC25560.1269975027799336No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG24950.12396665470889451No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG24930.12386728264099156No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG24910.12376791057308865No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24530.12187984128293314No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23790.11820307477052505No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23580.11715966805754437No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA22770.11313509930747606No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA22320.11089922777966033No Hit
GTCCAGGGACGCCTGTCATTTACATCAGAGAATAGTGTCCGGGGGGCTTG21990.10925958865926212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21950.10906084452345628No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21520.10692434506354347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21120.10493690370548504No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.075.4957661
GTATCAA55100.061.686671
ATCAACG54700.061.075473
TCAACGC55100.060.632094
TATCAAC56450.059.3096242
CAACGCA61650.054.307035
AACGCAG62450.053.4960486
CGCAGAG67250.049.1424188
ACGCAGA74300.044.3826077
GCAGAGT74550.044.1350029
GTACATG53150.028.5877571
TACATGG53250.028.1241822
CAGAGTA74700.027.8542739
ACATGGG54600.026.9013273
TATTGAG35250.026.5534155
GCCCATA10400.025.6194211
TAGACGG18600.025.5486035
ATAGACG18900.025.5240254
GTCTATT23400.024.6192651
TTATTGA38350.024.4069864
Produced by FastQC (version 0.11.5)