FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722746
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAAGGCGGTGTCCTCAGTTCTCAGACTGTTCAGTTGCAAATACA21060.29138867596638374No Hit
TTATATTACTGTGTAAAAGACAGGCCTAATGGGAGCATTGACTATTGGGG18770.25970396238789284No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT18670.25832035044123386No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT18420.2548613205745864No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA17700.2448993145586416No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14290.19771814717756997No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13800.19093844863894094No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT12710.17585707842035792No Hit
GTTCTCAGACTGTTCAGTTGCAAATACAGGGAGTTTTTGCTGTTGTCTCT12670.17530363364169432No Hit
GTCTGAGAACTGAGGACACCGCCTTATATTACTGTGTAAAAGACAGGCCT12590.17419674408436714No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12190.16866229629773113No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG12160.16824721271373344No Hit
GGTCTGGAGTGCGTCGCTGTTATTCATCGTGACGGTGGTCTCACAGGCTA12080.16714032315640626No Hit
ATATAAGGCGGTGTCCTCAGTTCTCAGACTGTTCAGTTGCAAATACAGGG11980.16575671120974728No Hit
ATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAA11560.1599455410337795No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG11030.15261239771648685No Hit
GCCTTATATTACTGTGTAAAAGACAGGCCTAATGGGAGCATTGACTATTG11000.15219731413248916No Hit
GTAAAAGACAGGCCTAATGGGAGCATTGACTATTGGGGCCAGGGAACCCT10710.14818483948717806No Hit
GTCTTTTACACAGTAATATAAGGCGGTGTCCTCAGTTCTCAGACTGTTCA10080.1394680842232264No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9950.13766938869256973No Hit
CTCCAGACCCTTTCCCGGAGCCTGACGGACCCAGTGCATATTATAACGAC9870.13656249913524252No Hit
CTCCTGTGCAGCCTCTGGATTCACGTTTGGTCGTTATAATATGCACTGGG9420.13033624537527708No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9420.13033624537527708No Hit
ATATTACTGTGTAAAAGACAGGCCTAATGGGAGCATTGACTATTGGGGCC9050.1252168811726388No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACGTTTGGTCGTTATAAT9000.1245250751993093No Hit
GTGTGAACCTTGCAGGCAGTAATAAATCGCAACATCCTCAGCCTCCACCC8820.12203457369532311No Hit
GGTTCACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAG8760.12120440652732772No Hit
GCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGTTCCACGACAC8750.12106604533266181No Hit
AATATGCACTGGGTCCGTCAGGCTCCGGGAAAGGGTCTGGAGTGCGTCGC8620.11926734980200514No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA8600.11899062741267334No Hit
CAGTAATATAAGGCGGTGTCCTCAGTTCTCAGACTGTTCAGTTGCAAATA8550.11829882143934384No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGGCACGGTCACCACGCTGCT8470.11719193188201664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8440.11677684829801895No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG8220.11373290201536915No Hit
TTATAATATGCACTGGGTCCGTCAGGCTCCGGGAAAGGGTCTGGAGTGCG8220.11373290201536915No Hit
GTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGTCGTGGTACAGCCGGGGG8160.11290273484737376No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8030.11110403931671707No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC7860.10875189900739679No Hit
CTTTTACACAGTAATATAAGGCGGTGTCCTCAGTTCTCAGACTGTTCAGT7800.1079217318394014No Hit
GCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGGTTTTC7770.10750664825540371No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGT7630.1055695915300811No Hit
GTCCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGA7610.10529286914074931No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7600.1051545079460834No Hit
GGCCTAATGGGAGCATTGACTATTGGGGCCAGGGAACCCTGGTCACCGTC7600.1051545079460834No Hit
GATCAGCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGG7550.10446270197275391No Hit
GGCTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAG7550.10446270197275391No Hit
GTCCACCACCACGCACGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT7540.10432434077808801No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCA7500.1037708959994244No Hit
GTGTAAAAGACAGGCCTAATGGGAGCATTGACTATTGGGGCCAGGGAACC7490.10363253480475852No Hit
GTCTGGGGGAGTCGTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTG7370.10197220046876773No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT7360.10183383927410182No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7330.10141875569010413No Hit
CATTAGGCCTGTCTTTTACACAGTAATATAAGGCGGTGTCCTCAGTTCTC7310.10114203330077233No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)