FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544104
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCGCAGCGGTTACAGAGGTCATCTTCAGGGAGA42040.27226145389170675No Hit
GTATATTACTGTGCGAGAGTCAAGTATTACGATTTTTGGAGTGGTTATGG40250.2606689704838534No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT32560.21086662556408117No Hit
CTATTATAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCA29700.19234455710237136No Hit
GTATTACTGTCAGCAGCATGGTAGCTCACTCTCGATCACCTTCGGCCAAG29030.18800547113406868No Hit
ATCTATTATAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT28050.18165874837446183No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27640.17900348681176914No Hit
TTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCAT22530.14590986099381906No Hit
ATTATAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACC22250.1440965116339314No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC21580.13975742566562874No Hit
GTCTCTGGTGGCTCCGTCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC21440.1388507509856849No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20920.13548310217446494No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18760.12149440711247429No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC18490.11974582022972546No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTGTGCTGATGGTGAGAGT17770.11508292187572859No Hit
GGTTATGGGTTAGGCTGGTTCGACCCCTGGGGCCAGGGAACCCTGCTCAC17400.1126867102215913No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17350.11236289783589706No Hit
TCTATGATGCATCCAGGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGC16540.10711713718765058No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16050.10394377580784714No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15910.1030371011279033No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15810.10238947635651484No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15800.102324713879376No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15460.10012278965665525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29500.060.9316831
TCAACGC30500.058.8460124
ATCAACG30650.058.558023
CAACGCA32550.055.13815
AACGCAG33500.053.2884756
TATCAAC35800.050.335512
CGCAGAG38550.046.676678
ACGCAGA41350.042.9937827
GGTATCA13950.041.3982161
GCAGAGT44600.040.344979
TATACGG20700.036.2131655
GTACATG44850.035.892881
TACGGCC21300.035.8187567
ATACGGC21100.035.4794856
TACATGG44800.035.0748632
ACATGGG44000.034.8934943
GTACACG10950.034.2288785
TACACGG11000.034.028056
ACGGGGT12100.030.9315859
ACACGGG12600.030.2753757
Produced by FastQC (version 0.11.5)