FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826314
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13450.16277105313476475No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13400.16216595628296265No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13220.1599876076164751No Hit
ATAATACACGGCCGTGTCTTCAAAAATAAGGCTGCTCAGCTCCATGTAGG12960.15684110398710419No Hit
GTATTATTGTGCGAGCAGATATTGTGTTAGTACCAGGTGCTCTGGAACCT11310.1368729078776349No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11270.1363888303961932No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10680.12924868754492844No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10670.12912766817456803No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCG10540.12755441635988257No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10510.12719135824880132No Hit
GCCTACATGGAGCTGAGCAGCCTTATTTTTGAAGACACGGCCGTGTATTA10050.12162446721222199No Hit
CAATAATACACGGCCGTGTCTTCAAAAATAAGGCTGCTCAGCTCCATGTA8690.1051658328432049No Hit
GTGTATTATTGTGCGAGCAGATATTGTGTTAGTACCAGGTGCTCTGGAAC8680.10504481347284446No Hit
GATATTGTGTTAGTACCAGGTGCTCTGGAACCTTTGACTTCTGGGGCCAG8530.10322952291743817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11400.063.155471
TCAACGC11700.060.8431744
CAACGCA12000.059.329295
ATCAACG12050.059.068783
AACGCAG12900.055.754276
CGCAGAG13650.053.217768
TATCAAC13700.052.4794542
ACGCAGA16400.043.8554957
CAGCGCT4600.037.5250479
GGTATCA4600.032.8683051
GAATACG2206.7848305E-1032.6922769
GATTGCG950.00491519530.283377
GTACATG21000.028.7988951
ACATGGG20350.028.6174183
AGCGCTG4400.027.7850675
ACGTTAA1050.00801411327.3992429
GCAGAGT29350.025.4854979
CAGAGTA18450.025.3387289
GGCAGCG6300.025.1159737
GTATAGG3902.1827873E-1123.9990771
Produced by FastQC (version 0.11.5)