FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799805
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTACACCGATCTCTCACACAGAAATACAGAGCCGTGTCCTCAGATCT23030.2879451866392433No Hit
TCTAAGAGGTGTCCAGTGTCAGGTGCAACTGGTGGAGTCTGGGGGAGGCG20370.2546870799757441No Hit
GTGTAGTAGTACCAGTTGTATATTACCCATTGATGCTTTTGATATGTGGG18320.22905583235913754No Hit
ATATACAACTGGTACTACTACACCGATCTCTCACACAGAAATACAGAGCC16010.20017379236188823No Hit
GTTGTATATTACCCATTGATGCTTTTGATATGTGGGGCCAAGGGACAATG12420.1552878514137821No Hit
GTATCTGCATATGAACAACCTGAGATCTGAGGACACGGCTCTGTATTTCT11260.14078431617706816No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11250.1406592857008896No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10930.1366583104631754No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10570.13215721332074692No Hit
TGCAGATACAGCGTGCTCTTGGAGTTGTCTCTGGAGATGGTGAACCGGCC10410.13015672570188982No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC10240.12803120760685416No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC9700.12127956189321148No Hit
TAACTATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGT9290.11615331236989015No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9060.11327761141778309No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8640.1080263314182832No Hit
GTATAGTGACTGGCCTCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGA8630.10790130094210464No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT8480.10602584379942612No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8400.10502559998999757No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8140.10177480760935478No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8020.10027444189521197No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG855.5384E-542.384871
ATCAACG15350.041.2924923
TCAACGC15400.041.1506964
CAACGCA15900.039.856655
AACGCAG16700.037.9473536
GTATCAA19050.036.6890531
TATCAAC17750.036.5208552
CGCAGAG18250.034.7157448
CTTACGG1051.9260179E-434.2986832
TGTTGCG1153.293625E-431.3024867
GCAGAGT21450.029.8723499
ATAGACG1753.970541E-628.8108963
TAGACGG1255.359923E-428.805494
TTACGAT1000.006276735628.805494
ACGCAGA22200.028.5388457
GTACATG19700.027.7975861
ACATGGG18750.026.5060233
GTACACG4950.026.1868115
TACATGG19950.025.9947932
GGTATCA13550.025.5247631
Produced by FastQC (version 0.11.5)