FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877890
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA51410.5856086753465696No Hit
GTGTATTACTGTGCGAGAGATTGGGCGGAGCATAGTAGATCCCTCTTTCC36340.4139470776520976No Hit
TTTAAGAGGTGTCCAGTGTCAGGCGCAGCTGGTGGAGTCTGGGGGAGGCG27020.30778343528232466No Hit
CTCCTGTACAGCCTCTGGATTCACCCTCAGAAACTATGCTATGCACTGGG26700.3041383316816458No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25500.2904691931790999No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21490.24479148868309242No Hit
AATAAATACTACGCAGACTCCGTGAGGGGCCGATTCACCATCTCCAGAGA21190.24137420405745594No Hit
ACTATGCTCCGCCCAATCTCTCGCACAGTAATACACAGCCGTGTCCTCAG19750.2249712378544009No Hit
CTACTATGCTCCGCCCAATCTCTCGCACAGTAATACACAGCCGTGTCCTC19120.21779494014056427No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC18450.21016300447664285No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17770.2024171593252002No Hit
GTATTTATTAATTCCATCATATGCTATAACTGCCACCCACTCCAGCCCCT17460.19888596521204252No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17420.19843032726195764No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC17180.19569649956144847No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15610.17781271002061763No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14680.16721912768114458No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14180.16152365330508378No Hit
GCATAGTAGATCCCTCTTTCCTATTAGTCAAATGGACGTCTGGGGCCAAG13900.15833418765448976No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13630.15525863149141694No Hit
ATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAGCG13520.15400562712868354No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13290.15138570891569558No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13240.15081616147808952No Hit
ATACTACGCAGACTCCGTGAGGGGCCGATTCACCATCTCCAGAGACAATT13110.14933533814031372No Hit
GTATAGTGACTGGCCTCCGCTCACCTTCGGCGGAGGGACCAAGGTGGAGA12930.14728496736493182No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12860.14648760095228333No Hit
GTACAGGAGAGTCTCAGGGACCTCCCAGGCTGGACCACGCCTCCCCCAGA12680.14443723017690144No Hit
GTATTACTGTGCGAGAGATTGGGCGGAGCATAGTAGATCCCTCTTTCCTA12630.14386768273929534No Hit
GTCCAGTGTCAGGCGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC12290.13999476016357404No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA12150.138400027338277No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12080.1376026609256285No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12020.1369192040005012No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGTTTC11530.13133763911196164No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11260.1282620829488888No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11200.12757862602376152No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT11050.1258699837109433No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC10900.12416134139812504No Hit
GTTATAGCATATGATGGAATTAATAAATACTACGCAGACTCCGTGAGGGG10890.12404743191060383No Hit
GGGCTGGAGTGGGTGGCAGTTATAGCATATGATGGAATTAATAAATACTA10710.12199706113522196No Hit
GAGTACACACTCACAATCAGCAGCCTACAGTCTGAAGATTTTGCAGTTTA10700.12188315164770074No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC10610.1208579662600098No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT10600.12074405677248858No Hit
GAGCATAGTAGATCCCTCTTTCCTATTAGTCAAATGGACGTCTGGGGCCA10070.11470685393386415No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT9980.11368166854617322No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9960.11345384957113078No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG9930.11311212110856714No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGTACACCAGTGGCCCTGGT9930.11311212110856714No Hit
CCCTGAGACTCTCCTGTACAGCCTCTGGATTCACCCTCAGAAACTATGCT9910.1128843021335247No Hit
GCTGTGTATTACTGTGCGAGAGATTGGGCGGAGCATAGTAGATCCCTCTT9880.11254257367096106No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC9870.11242866418343983No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9740.11094784084566403No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG9690.11037829340805796No Hit
GCGTAGTATTTATTAATTCCATCATATGCTATAACTGCCACCCACTCCAG9660.1100365649454943No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG9630.10969483648293067No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9490.10810010365763364No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC9310.10604973288225177No Hit
CTCCGTGAGGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAGCACGC9170.10445500005695474No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA8960.10206290081900922No Hit
GTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACT8910.10149335338140314No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)