FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765788
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGAAACA65480.8550669375858593No Hit
GTCTATTACTGTGCGAGAGGTCGGGATGGCTACAATCCAGACTTTGACTA47510.6204066921915726No Hit
ATATTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG24240.31653669161700104No Hit
ATATGGGACTACTCCTACTAGTAATATATGAAAGCCACTCCAGCCCCTTC20970.2738355785152026No Hit
GCGTAATATATGGGACTACTCCTACTAGTAATATATGAAAGCCACTCCAG19640.25646784749826324No Hit
CCCATATATTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA18390.24014479203121492No Hit
ATATATGGGACTACTCCTACTAGTAATATATGAAAGCCACTCCAGCCCCT17270.2255193343327396No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGGCCAGTGTGAGGTGC17240.22512758100153044No Hit
GTCCCATATATTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGA17000.2219935543518572No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15360.20057770557908977No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15070.19679075671073457No Hit
GTGTGAGGTGCAGTTGGTGGAGTCTGGGGGAGGCTTAGTACAGCCTGGGG14850.19391789894853406No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14620.19091445674259716No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14360.18751926120545112No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12930.16884568575114783No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA12530.16362230800169236No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12370.1615329569019102No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG12360.1614023724581738No Hit
AGTAGGAGTAGTCCCATATATTACGCAGACTCTGTGAAGGGCCGATTCAC12260.16009652802080993No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC12230.1597047746896008No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC12080.15774600803355499No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGGCCA12020.15696250137113665No Hit
ACTCCTACTAGTAATATATGAAAGCCACTCCAGCCCCTTCCCTGGAGCCT11980.15644016359619112No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC11790.15395905916519978No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11220.14651574587222574No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA10960.1431205503350797No Hit
GCTTAGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGA10790.14090061479156113No Hit
GTACTAAGCCTCCCCCAGACTCCACCAACTGCACCTCACACTGGCCACCT10660.13920301702298807No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATGAAATGAACTGGG10650.1390724325792517No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10320.13476314593595093No Hit
CTCCAGAGACAACGCCAAGAACTTACTGTTTCTGCAAATGAACAGCCTGA10150.13254321039243236No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATGAA10140.132412625948696No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9810.12810333930539522No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9740.12718924819924052No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA9720.12692807931176775No Hit
GTACATGGGAGCTCTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATT9340.12196587044978506No Hit
GTCTGGGGGAGGCTTAGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTG9290.12131294823110313No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG9250.12079061045615759No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA9220.12039885712494841No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGA9210.12026827268121203No Hit
GAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTCCTTGT9200.12013768823747564No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC9190.12000710379373926No Hit
CAGTAATAGACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGAAA9130.11922359713132094No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG9110.11896242824384817No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8830.11530606381922935No Hit
GAGTAGTCCCATATATTACGCAGACTCTGTGAAGGGCCGATTCACCATCT8660.11308612827571078No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8570.11191086828208328No Hit
GTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGC8070.10538164609526397No Hit
CTATTACTGTGCGAGAGGTCGGGATGGCTACAATCCAGACTTTGACTACT7950.10381463277042732No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA7850.10250878833306346No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7850.10250878833306346No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG7780.10159469722690875No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG7770.10146411278317237No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT7670.1001582683458085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)