FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765788
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGAAACA63220.825554853301436No Hit
GTCTATTACTGTGCGAGAGGTCGGGATGGCTACAATCCAGACTTTGACTA50400.6581455964313883No Hit
ATATTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG23410.305698182786881No Hit
ATATGGGACTACTCCTACTAGTAATATATGAAAGCCACTCCAGCCCCTTC20000.2611688874727731No Hit
GCGTAATATATGGGACTACTCCTACTAGTAATATATGAAAGCCACTCCAG19090.24928570309276196No Hit
GTCCCATATATTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGA19010.2482410275428709No Hit
CCCATATATTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA17960.23452966095055028No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGGCCAGTGTGAGGTGC17420.2274781009887854No Hit
ATATATGGGACTACTCCTACTAGTAATATATGAAAGCCACTCCAGCCCCT16720.21833718992723836No Hit
GTGTGAGGTGCAGTTGGTGGAGTCTGGGGGAGGCTTAGTACAGCCTGGGG15250.1991412766979895No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14060.18360172789335952No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12880.16819276353246593No Hit
AGTAGGAGTAGTCCCATATATTACGCAGACTCTGTGAAGGGCCGATTCAC12730.1662339968764201No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12340.16114120357070102No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC12290.16048828135201912No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA12270.16022711246454632No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12230.1597047746896008No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG12200.1593130213583916No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA12100.15800717692102775No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGGCCA11900.15539548804630002No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11860.15487315027135445No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11770.15369789027772698No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11700.1527837991715723No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10920.14259821256013414No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATGAAATGAACTGGG10860.14181470589771583No Hit
CTCCAGAGACAACGCCAAGAACTTACTGTTTCTGCAAATGAACAGCCTGA10690.13959477035419726No Hit
GCTTAGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGA10680.13946418591046084No Hit
ACTCCTACTAGTAATATATGAAAGCCACTCCAGCCCCTTCCCTGGAGCCT10620.13868067924804253No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA10440.1363301592607876No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATGAA10360.1352854837108965No Hit
GTACTAAGCCTCCCCCAGACTCCACCAACTGCACCTCACACTGGCCACCT10190.13306554816737792No Hit
GAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTCCTTGT10040.13110678151133212No Hit
GTACATGGGAGCTCTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATT10020.13084561262385935No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA9990.1304538592926502No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG9840.12849509263660439No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC9790.12784217041792245No Hit
GTCTGGGGGAGGCTTAGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTG9690.12653632598055858No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC9270.12105177934363036No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC9230.1205294415686848No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9180.11987651935000287No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG9160.11961535046253009No Hit
CTATTACTGTGCGAGAGGTCGGGATGGCTACAATCCAGACTTTGACTACT9070.11844009046890261No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC8950.11687307714406599No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8840.11543664826296574No Hit
CAGTAATAGACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGAAA8830.11530606381922935No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8760.11439197271307464No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG8710.1137390504943927No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGA8630.1126943749445016No Hit
GAGTAGTCCCATATATTACGCAGACTCTGTGAAGGGCCGATTCACCATCT8530.11138853050713775No Hit
CCCTCATGCTGCCACCCCCGACTGTCACTGCACCGACCGGCCCTCGAGGA8520.11125794606340136No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT8160.10655690608889146No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7750.1012029438956996No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7730.10094177500822683No Hit
GGACTACTCCTACTAGTAATATATGAAAGCCACTCCAGCCCCTTCCCTGG7720.10081119056449042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC9000.067.21584
ATCAACG9400.064.368173
CAACGCA9400.063.5811045
GAATACG2400.062.9869
TATCAAC9700.061.6388472
AACGCAG9950.060.0469476
GTATCAA10550.058.736451
CGCAGAG11250.052.4683348
GTGCTAG3550.050.742681
ACAGCCG21150.046.2875828
CAGCCGT21600.045.6565139
TAGACAG22650.043.8721285
GTAATAG24300.042.40251
ATAGACA23350.042.2540444
AATAGAC23500.041.6860543
GACAGCC24050.041.0054367
TAATAGA24200.040.4829062
GCAGAGT14750.040.018229
AGACAGC25250.039.911926
GGTATCA3250.039.907161
Produced by FastQC (version 0.11.5)