FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1998791
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTC148220.7415482659267527No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC98150.4910468378134582No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAATTT86390.43221127171375096No Hit
TCTATGAGGACAGTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC73510.3677723183664525No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT70040.3504118239475763No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA68980.3451086181596775No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA63270.316541349245619No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT58960.2949783143910494No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT58420.29227668125381795No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC57230.2863230823032523No Hit
GTAATAGACGGCCGTGTCTTCAGGACTCAGGCTACTCAACTTCATGTAGA53510.2677118318023245No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACG53360.26696137815309356No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA52440.2623585957711437No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG50590.2531030007639618No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC49490.24759967400293476No Hit
GTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG48220.24124583310611267No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG47140.23584256683164972No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA46950.23489199220929052No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG46860.23444172001975191No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46740.23384135710036716No Hit
GTCTATTACTGTGCGAGAGATGTGATTGGCAGCAGCAGCTGGCTGGTGGG46430.23229041955862317No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG46400.232140328828777No Hit
CCCCTCTACTGGTCATCTATGAGGACAGTAAACGACCCTCCGGGATCCCT46330.23179011712580253No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA44320.22173403822610768No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG44080.22053331238733814No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA43940.21983288898138925No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT43550.21788170949338875No Hit
GTAGTAGTCAGCTTCATCCTCAACCTGGGCCCCACTGATAGTCAAGGTGG42980.21502998562631112No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCC42940.21482986465318285No Hit
GTAGAGGGGCCTGGCCTGACTTCTGCTGGAACCAGTAAGAATATTTTTTT42320.21172798956969485No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC41450.20737535840415533No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA41430.2072752979175912No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCG39790.1990703380193327No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT39770.19897027753276855No Hit
CTACTGGTCATCTATGAGGACAGTAAACGACCCTCCGGGATCCCTGAGAG38280.19151577128374103No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT38160.19091540836435625No Hit
CAGTAGTAGTCAGCTTCATCCTCAACCTGGGCCCCACTGATAGTCAAGGT37920.18971468252558674No Hit
CTACTACTGTTACTCAACAGACAGCAGTGGTAATTATAAATTATTCGGCG37550.18786356352415035No Hit
GACTACTACTGTTACTCAACAGACAGCAGTGGTAATTATAAATTATTCGG37380.18701304938835525No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATTCTTACTGGTTCCAGCAGAAG35350.17685691000209627No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA34760.17390512564845448No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG34560.1729045207828132No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAATTTATAATTACCA33820.16920228277994048No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC32650.163348744315939No Hit
GTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCC32370.16194789750404118No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAATTTATAATTACCAC32310.1616477160443488No Hit
GTCTACATGAAGTTGAGTAGCCTGAGTCCTGAAGACACGGCCGTCTATTA32140.16079720190855373No Hit
CCTCATAGATGACCAGTAGAGGGGCCTGGCCTGACTTCTGCTGGAACCAG32010.16014680874588688No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG32000.16009677850260484No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC31860.15939635509665595No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC31610.15814559901460434No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC30730.1537429376057827No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT29390.14703888500598614No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA28850.14433725186875465No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC28750.14383694943593403No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG28560.1428863748135748No Hit
GACTATCAGTGGGGCCCAGGTTGAGGATGAAGCTGACTACTACTGTTACT27680.13848371340475316No Hit
GTATCAACGCAGAGTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAA27660.13838365291818905No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA27520.13768322951224016No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA27460.13738304805254775No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27090.1355319290511114No Hit
ACCTGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCC26620.13318050761685438No Hit
TCCTCAACCTGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCCTGAGCT26310.1316295700751104No Hit
CTCTACTGGTCATCTATGAGGACAGTAAACGACCCTCCGGGATCCCTGAG26240.13127935837213595No Hit
CATGTAGACTGTCCTCGTGGACGTGTCCCTGGTCATATTGACTCTGCCCT25670.1284276345050583No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG25470.12742702963941704No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25470.12742702963941704No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA25260.12637639453049368No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25180.12597615258423717No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT24780.12397494285295461No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC24740.12377482187982634No Hit
GGTTGAGGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTA24690.12352467066341605No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG23910.11962231168741505No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTC23880.11947222095756885No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT23830.11922206974115852No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC23440.11727089025315802No Hit
TACCACTGCTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCAACC23250.11632031563079882No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC23230.11622025514423469No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT22830.11421904541295212No Hit
CCTTGACTATCAGTGGGGCCCAGGTTGAGGATGAAGCTGACTACTACTGT22720.11366871273684942No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22630.11321844054731085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22550.11281819860105434No Hit
CTTCATACAGTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG22420.1121678054383875No Hit
GTCGTTTACTGTCCTCATAGATGACCAGTAGAGGGGCCTGGCCTGACTTC22140.11076695862648972No Hit
ACAGACAGCAGTGGTAATTATAAATTATTCGGCGGAGGGACCAAGCTGAC21880.10946617230115606No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA21640.10826544646238652No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC21600.10806532548925825No Hit
GCTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCAACCTGGGCCC21580.10796526500269413No Hit
TAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGG21540.10776514402956587No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA21530.1077151137862838No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA21190.10601408551469363No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21140.1057639342982833No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG20840.1042630269998214No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20820.10416296651325725No Hit
GCAGTGGTAATTATAAATTATTCGGCGGAGGGACCAAGCTGACCGTCCTG20330.10171148459243613No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20150.10081094021335897No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33000.086.869861
GGTATCA11900.085.9491041
ATCAACG42550.066.670483
TCAACGC42800.066.281064
TATCAAC43450.065.4552152
CAACGCA45650.062.458475
AACGCAG47700.059.77426
CGCAGAG52700.053.8284238
ACGCAGA60400.046.9661987
CAGCGCT7150.040.2791259
GTACATG56650.038.1435661
TACATGG57500.036.563852
ACATGGG58350.035.784433
TATTGAG53100.035.3901945
TAGACGG21250.034.8991785
AATAGAC22450.034.6373253
TTATTGA54900.034.6233064
CATGGGG42450.034.6010634
AGACGGC21250.034.5603526
GCAGAGT83850.034.346549
Produced by FastQC (version 0.11.5)