FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492284
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA213500.8566439458745473No Hit
CTCATATACAGGTCGTGGCGCCCTTTATGTCTTCGGAACCGGGACCAAGG110170.4420443256065521No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTCGTGTGTCTGATCAGT105180.4220225303376341No Hit
GCTCATATACAGGTCGTGGCGCCCTTTATGTCTTCGGAACCGGGACCAAG103040.4134360289597815No Hit
GTCCTGCACTGGAACCAGCAGTGATATTGGGAATTTTAACTTTGTCTCCT91460.36697262430766314No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC90600.36352197422123644No Hit
CTCCTGGACAGTCGATCACCATGTCCTGCACTGGAACCAGCAGTGATATT86910.3487162779201728No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT81470.3268889099316129No Hit
GATTATTACTGCTGCTCATATACAGGTCGTGGCGCCCTTTATGTCTTCGG78630.3154937398787618No Hit
TATACAGGTCGTGGCGCCCTTTATGTCTTCGGAACCGGGACCAAGGTCAC77870.3124443281744777No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA77300.31015726939626465No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG73150.29350587653734483No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC69330.27817857033949583No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA68080.27316309056271276No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC65760.26385436009700336No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTCGTGT63160.2534221621612946No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC62090.24912891147236832No Hit
ACCTGTATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC56060.22493423702916682No Hit
GCCTTGGGCTGACCGAGGACGGTGACCTTGGTCCCGGTTCCGAAGACATA55440.22244655905988242No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA53470.2145421629316723No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT53010.21269646637381615No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG52860.21209460880060216No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG51910.20828284417024706No Hit
GTCGATCACCATGTCCTGCACTGGAACCAGCAGTGATATTGGGAATTTTA51690.20740011972953323No Hit
CATATACAGGTCGTGGCGCCCTTTATGTCTTCGGAACCGGGACCAAGGTC47220.189464764047757No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT45370.18204185397811806No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC41860.1679583867649112No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC38450.15427615793384702No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG36640.14701374321706515No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCT36260.1454890373649231No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCC36100.14484705595349487No Hit
CTTTATGTCTTCGGAACCGGGACCAAGGTCACCGTCCTCGGTCAGCCCAA36030.14456618908599503No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG35640.1430013593956387No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG35460.14227913030778194No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT33310.13365250509171508No Hit
CTCGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG32600.1308037125785023No Hit
CTGTATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCA32250.12939937824100303No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA31010.12442402230243423No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC31000.12438389846421997No Hit
TCCTCGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC30830.12370179321457749No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC30770.1234610501852919No Hit
GACATAAAGGGCGCCACGACCTGTATATGAGCAGCAGTAATAATCAGCCT30460.12221721120064968No Hit
GTACATGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATG30440.12213696352422117No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA30160.12101349605422175No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT30150.1209733722160075No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG29330.11768321748243779No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGAC28930.11607826395386722No Hit
GCACTGGAACCAGCAGTGATATTGGGAATTTTAACTTTGTCTCCTGGTAC28590.11471405345458222No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG28410.11399182436672545No Hit
GGCCACACTCGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG28110.11278810922029754No Hit
GTATCAACGCAGAGTACATGGGGGGGTCACAAGAGGCAGCGCTCTCGGGA28080.11266773770565475No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGGTGACCTTGGTC27840.11170476558851239No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACGAGTGTGGCC27750.111343651044584No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG27720.11122327952994121No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27670.11102266033886987No Hit
GATCAGACACACGAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG27630.11086216498601284No Hit
TTATTACTGCTGCTCATATACAGGTCGTGGCGCCCTTTATGTCTTCGGAA26340.1056861898563727No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT26300.10552569450351565No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCC26260.10536519915065859No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25880.10384049329851654No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA25630.10283739734315994No Hit
ACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTC25170.10099170078530376No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATAT25070.10059046240316111No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATGT25040.10047009088851831No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG24950.10010897634458996No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGG24950.10010897634458996No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43450.086.369971
GGTATCA18400.070.8558351
TCAACGC54700.067.657564
ATCAACG55300.066.9234853
TATCAAC57400.064.60052
CAACGCA58600.063.154765
AACGCAG60300.061.3718156
CGCAGAG70000.052.7645768
ACGCAGA71850.051.4059877
GCAGAGT77250.047.905769
GTACATG72450.034.5983731
ACATGGG74050.033.2538223
TACATGG73850.033.2463872
CATGGGG54600.031.3851974
ATATGAG105950.029.1539153
GTATATG108900.029.1031271
TATGAGC105250.029.0057624
ATGAGCA109050.028.0610455
TATATGA110850.027.8651982
TGAGCAG122800.025.0939226
Produced by FastQC (version 0.11.5)