FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492284
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA196170.7871093342492268No Hit
CTCATATACAGGTCGTGGCGCCCTTTATGTCTTCGGAACCGGGACCAAGG94930.38089559616801294No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTCGTGTGTCTGATCAGT93010.3731918192308742No Hit
GCTCATATACAGGTCGTGGCGCCCTTTATGTCTTCGGAACCGGGACCAAG88030.35321014780017046No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT79210.3178209224951892No Hit
GTCCTGCACTGGAACCAGCAGTGATATTGGGAATTTTAACTTTGTCTCCT78750.31597522593733296No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC76170.30562327567805275No Hit
CTCCTGGACAGTCGATCACCATGTCCTGCACTGGAACCAGCAGTGATATT76160.3055831518398385No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA72090.28925274968663284No Hit
TATACAGGTCGTGGCGCCCTTTATGTCTTCGGAACCGGGACCAAGGTCAC70340.28223107799913655No Hit
GATTATTACTGCTGCTCATATACAGGTCGTGGCGCCCTTTATGTCTTCGG69540.27902117094199536No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG68010.2728822236952129No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA63660.25542835407200787No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC61240.24571838522415584No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC57550.23091268892309222No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC55830.22401138875023874No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTCGTGT53910.21630761181309996No Hit
ACCTGTATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC52710.2114927512273882No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG51930.20836309184667556No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT49220.1974895316906099No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA48980.19652655957346754No Hit
GTCGATCACCATGTCCTGCACTGGAACCAGCAGTGATATTGGGAATTTTA47850.19199256585525565No Hit
GCCTTGGGCTGACCGAGGACGGTGACCTTGGTCCCGGTTCCGAAGACATA47270.1896653832388283No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG46250.18557275174097335No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT42340.16988433099919592No Hit
CATATACAGGTCGTGGCGCCCTTTATGTCTTCGGAACCGGGACCAAGGTC41920.1681991297941968No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC38220.15335330965491895No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC34730.13935009011814065No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG34120.1369025359870705No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG33210.13325126670957244No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG32250.12939937824100303No Hit
CTTTATGTCTTCGGAACCGGGACCAAGGTCACCGTCCTCGGTCAGCCCAA32100.12879752066778907No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCC32050.12859690147671773No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCT31580.1267110810806473No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT31510.12643021421314746No Hit
CTGTATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCA30030.12049188615743632No Hit
CTCGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG29680.11908755181993705No Hit
GACATAAAGGGCGCCACGACCTGTATATGAGCAGCAGTAATAATCAGCCT28660.11499492032208207No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC28310.11359058598458283No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG27880.11186526094136943No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG27540.11050105044208446No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG27510.11038067892744166No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT27400.10993931670708476No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC27070.10861523004601402No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA26430.10604730440030108No Hit
TCCTCGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC26220.10520470379780154No Hit
GCACTGGAACCAGCAGTGATATTGGGAATTTTAACTTTGTCTCCTGGTAC26150.10492383693030169No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA26070.10460284622458757No Hit
GTACATGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATG26010.10436210319530198No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25850.10372012178387374No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGGTGACCTTGGTC25780.10343925491637389No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGAC25720.1031985118870883No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)