FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953452
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC57910.2964495672276565No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTG44130.2259077776162404No Hit
GTCCTGGGCCCACTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC38640.19780368291619144No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTT36390.18628561131781074No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36010.18434033700341754No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAG35040.1793747683587823No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30970.15853985662304476No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA27560.1410835792228322No Hit
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTAGCAGATACA26570.1360156277195447No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25820.13217627052008446No Hit
CTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATGTCTCCT25680.13145959050951855No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA25340.1297190819124299No Hit
GGTTATAACTATGTCTCCTGGTACCAACAAACCCCAGGCAAAGCCCCCAA24860.12726189330477533No Hit
GTGTATTACTGTGCGAAAGATCTCCGATACAGTTATGGTTACGGGGCTAT24740.12664759615286172No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT24740.12664759615286172No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC24390.12485589612644692No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24160.12367849325194578No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT23720.12142607036159578No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22610.11574382170639462No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21900.11210923022423892No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTGC20470.10478885583060141No Hit
TCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCACTCTGCCCTG20410.1044817072546446No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20260.10371383581475255No Hit
GTATATGAGCTGCAGAAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA20180.10330430438014347No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA20140.10309953866283891No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA19990.10233166722294687No Hit
GCTCATATACAAGCACCAACACTCTGGTGTTCGGCGGAGGGACCAAACTG19980.10228047579362073No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19630.10048877576720595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38050.076.119421
ATCAACG44850.063.103583
TCAACGC45000.062.8932344
GGTATCA18300.061.0245631
CAACGCA46950.060.2795145
TATCAAC48450.058.859182
AACGCAG48500.058.495556
ACGCAGA58800.048.245177
CGCAGAG60550.046.8508038
GCAGAGT77150.036.9567879
GTACATG57700.031.4665261
TACATGG58550.030.2566592
ACATGGG60950.028.3570753
CAGAGTA63550.024.6988039
TATTGAG29250.024.1275375
CCTAGGT36650.023.578712
TTATTGA32000.023.4049264
TAGGTCA38300.022.7515244
GTATATA9900.022.5605981
CATGGGG40300.022.5159054
Produced by FastQC (version 0.11.5)