FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953452
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC49110.25140110942065635No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTG37610.19253096569559938No Hit
GTCCTGGGCCCACTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC34310.17563779401797433No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTT32070.16417091384891977No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30770.157516028036522No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAG30090.15403501084234472No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27760.14210740780935494No Hit
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTAGCAGATACA25330.12966789048310376No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24400.12490708755577307No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA24180.12378087611059806No Hit
CTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATGTCTCCT23100.11825220174337531No Hit
GGTTATAACTATGTCTCCTGGTACCAACAAACCCCAGGCAAAGCCCCCAA22940.11743313887415713No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA22590.11564143884774236No Hit
GTGTATTACTGTGCGAAAGATCTCCGATACAGTTATGGTTACGGGGCTAT22210.11369616453334916No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT22040.11282591023480484No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC21390.10949846732860598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21360.10934489304062756No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT21020.10760438444353893No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20880.10688770443297302No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20850.10673413014499461No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36400.075.359551
ATCAACG42350.063.7616163
TCAACGC43150.062.2457164
CAACGCA45600.058.743465
GGTATCA17000.057.174131
TATCAAC47850.056.583182
AACGCAG48150.055.6324356
ACGCAGA55050.048.528637
CGCAGAG55300.048.4394578
GCAGAGT70600.038.1352049
GTACATG55050.030.3420831
TACATGG58200.028.4568162
ACATGGG59000.026.9725323
TATTGAG25400.026.932175
CAGAGTA58550.025.9426739
TTATTGA27350.025.5385274
GTAATAC17700.024.8125441
CTGTGCG19650.023.4464249
CAGCCGT18400.023.083069
TACGACG1952.7392714E-422.1563435
Produced by FastQC (version 0.11.5)