FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704132
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15270.2168627473257855No Hit
ATATAATAGTAATCTTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCA13090.1859026432543898No Hit
TTATTACTGTCAGCAATATAATAGTAATCTTCCCACTTTCGGCGGAGGGA12800.18178409729993808No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12370.1756772877812683No Hit
GTAAAACACAGCCGTGTCCTCGGGAGTCACAGAGTTCAGATGCAGGGAGA11460.16275357461385082No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT11030.15664676509518102No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT10870.1543744638789318No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA10220.1451432401879193No Hit
CCCATGGGGTGTCCTGTCACAGGTACACCTGCAGCAGTCAGGTCCAGGAC10160.14429112723182586No Hit
ACCTAGACACATCCACGAACCAGCTCTCCCTGCATCTGAACTCTGTGACT9960.14145075071151433No Hit
GCTTAGGAGGCTGCCCCGGTTTCTGCTGGTACCAAGCTAAGAGGTTCCTA9270.13165145171643952No Hit
GTCTATAGCACTGATGTTGCTTGGAACTGGATCAGGCAGTCCCCATCGAG9160.1300892446302682No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8660.12298830332948935No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8460.12014792680917782No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8160.11588736202871053No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT7970.11318900433441458No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT7790.1106326654661342No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT7660.1087864207279317No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7630.10836036424988497No Hit
GTCCAGGACTGGTGAAGCCCTCGCAGACCCTCTCACTCACCTGTGCCATC7590.10779228894582266No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7220.10253759238324632No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14800.056.9407731
TCAACGC14900.056.0712244
ATCAACG15450.054.075163
CAACGCA15450.053.5899625
TATCAAC16350.051.098542
AACGCAG16400.050.9246676
CGCAGAG18600.044.514248
TACACCG1151.4911893E-743.8242265
GGTATCA7650.041.427661
ACGCAGA21550.038.4179157
TAGTACG950.004881590630.3252724
GTACATG21000.030.1830121
ACATGGG19800.029.8275093
TACATGG21350.029.0114142
GCAGAGT29350.028.9459639
GCTAGTA1000.006272490628.8090082
GAATACG2303.7283826E-828.1727169
GTATACT1907.5281077E-626.5346134
CTATACC2301.1676784E-625.0513114
TAGGGGT8600.024.2866654
Produced by FastQC (version 0.11.5)