FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704132
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15240.2164366908477388No Hit
ATATAATAGTAATCTTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCA12760.18121602199587578No Hit
TTATTACTGTCAGCAATATAATAGTAATCTTCCCACTTTCGGCGGAGGGA12420.17638738191134617No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT11930.1694284594365829No Hit
GCTTAGGAGGCTGCCCCGGTTTCTGCTGGTACCAAGCTAAGAGGTTCCTA10940.15536859566104083No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10530.14954582379440218No Hit
GTAAAACACAGCCGTGTCCTCGGGAGTCACAGAGTTCAGATGCAGGGAGA10380.14741554140416852No Hit
CCCATGGGGTGTCCTGTCACAGGTACACCTGCAGCAGTCAGGTCCAGGAC9960.14145075071151433No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA9360.1329296211505797No Hit
ACCTAGACACATCCACGAACCAGCTCTCCCTGCATCTGAACTCTGTGACT9220.13094135758636166No Hit
GTCTATAGCACTGATGTTGCTTGGAACTGGATCAGGCAGTCCCCATCGAG9180.13037328228229936No Hit
CTATTATATTGCTGACAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCT8930.12682281163190992No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT8760.12440849158964512No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8400.11929581385308437No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8270.11744956911488187No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8140.11560332437667938No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT8050.11432515494253917No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC7990.11347304198644573No Hit
CAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT7610.1080763265978538No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7560.10736623246777592No Hit
GTCCAGGACTGGTGAAGCCCTCGCAGACCCTCTCACTCACCTGTGCCATC7360.1045258559474644No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT7300.10367374299137093No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG1553.6903657E-837.15619
GTGCTAG2800.036.0180051
TCAACGC12750.035.5892034
ATCAACG13250.034.248653
CAACGCA13500.033.07855
GTATCAA13750.033.005591
CCGTATG900.003755102931.995539
TATCAAC14200.031.9573652
AACGCAG14200.031.4366976
TGCACCG950.00488051430.326645
GGGTACG4850.029.705571
CGCAGAG15150.029.4612338
TACTCGT1000.006282002728.800077
TCTACAC5600.028.297853
CTATACT3403.6379788E-1227.5392654
TAGACCC1607.137114E-527.0096634
TACGTGC5350.026.9255224
GTACATG22150.025.69231
ACACAGT7050.025.5319776
ACGCAGA17850.025.0084657
Produced by FastQC (version 0.11.5)