FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705658
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCCCGGCGCTCAGGCTGTCCATTTGAAGATAGA60720.8604734871566679No Hit
ATTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGGCT25050.35498782696433684No Hit
ATATACAGCCGTGTCCCCGGCGCTCAGGCTGTCCATTTGAAGATAGAAGG20150.2855490903525504No Hit
GAATAGGACGTGTCACCAGCAGCACCAATGACTGAGACCCATTCCAGACC18900.2678351269311763No Hit
GTATATTACTGTGCAAGAGATCCAAGGGGGGGGGGGCCCACTTTTGACTA16930.23991792057909073No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13910.19712098495305092No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13410.19003539958450127No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC12730.18039900348327378No Hit
ATATTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGG12450.17643107567688596No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC12280.1740219766515791No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12070.17104603079678826No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA12060.17090431908941725No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11110.15744170688917294No Hit
CCTATGAGGTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA10890.1543240493270111No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC10740.1521983737164462No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10510.14893900444691338No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA10400.14738017566583245No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC10310.14610477029949354No Hit
CTCCTGTGCAGCCTCTGGATTCAGCATCAGTAGGTACGACATGCACTGGG10070.1427036893225897No Hit
GTCCTATTCAGTCTCCGTGAAGGGCCGATTCACCATCTCCAGAGAAACTG9840.13944432005305687No Hit
GATCAGGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGG9750.1381689146867179No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA9710.13760206785723395No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAGCCACCCCAGTGGTT9610.13618495078352402No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9530.1350512571245561No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9420.13349242834347516No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCATCAGTAGGTACGAC9350.13250044639187822No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8980.12725711321915148No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTT8870.12569828443807055No Hit
GTACATGGGAGCTCTGGGAGTGGAGCCCCAGCCTTGGGATTCCCAAGTGT8810.12484801419384461No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAGCCAC8650.12258062687590873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8590.12173035663168277No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG8560.12130522150956978No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8530.12088008638745681No Hit
GTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGGCTTGGTACAGCCGGGGG8200.11620360004421404No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7960.11280251906731023No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7740.10968486150514838No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7740.10968486150514838No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG7630.10812603272406746No Hit
GTATATTACTGTGCAAGAGATCCAAGGGGGGGGGGCCCACTTTTGACTAC7580.1074174741872125No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7550.10699233906509953No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7540.10685062735772853No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7420.10515008686927663No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7410.10500837516190563No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13700.065.181491
ATCAACG14900.057.9945683
TCAACGC14950.057.800614
TATCAAC15600.055.8538482
CAACGCA15900.054.792245
GTAATAT17350.054.7895281
AATATAC17550.054.5716173
AACGCAG16400.053.5607646
CAGCCGT17850.051.626399
TATACAG19150.050.3810045
ACGCAGA18200.047.8645557
ATATACA21400.047.445794
CGCAGAG18850.046.214058
ATACAGC21350.045.5267526
GAATACG1202.0837251E-741.996869
GCAGAGT21350.040.8025679
TAATATA25200.039.148252
GTACATG16650.038.494521
TACATGG16950.036.5360072
TATTACT14050.035.3643044
Produced by FastQC (version 0.11.5)