FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705658
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCCCGGCGCTCAGGCTGTCCATTTGAAGATAGA58210.8249038486065489No Hit
ATTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGGCT24310.34450116061888336No Hit
ATATACAGCCGTGTCCCCGGCGCTCAGGCTGTCCATTTGAAGATAGAAGG19630.2781800815692588No Hit
GAATAGGACGTGTCACCAGCAGCACCAATGACTGAGACCCATTCCAGACC18750.2657094513206114No Hit
GTATATTACTGTGCAAGAGATCCAAGGGGGGGGGGGCCCACTTTTGACTA15440.21880287618081282No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13490.1911690932434692No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA12830.1818161205569837No Hit
ATATTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGG12640.17912359811693485No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC11810.16736152640514243No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11800.16721981469777145No Hit
CTCCTGTGCAGCCTCTGGATTCAGCATCAGTAGGTACGACATGCACTGGG11440.16211819323241572No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10960.15531603127860805No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10830.15347377908278514No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10790.15290693225330115No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA10650.15092296835010727No Hit
CCTATGAGGTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA10570.14978927469113934No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG10300.14596305859212252No Hit
GTCCTATTCAGTCTCCGTGAAGGGCCGATTCACCATCTCCAGAGAAACTG10300.14596305859212252No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10150.14383738298155765No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA10100.14312882444470268No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAGCCACCCCAGTGGTT9970.14128657224887978No Hit
GATCAGGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGG9560.13547639224666908No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCATCAGTAGGTACGAC9370.13278386980662021No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC9350.13250044639187822No Hit
GTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGGCTTGGTACAGCCGGGGG9210.13051648248868433No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC9080.1286742302928614No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG8960.1269736898044095No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTT8850.12541486102332858No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG8660.12272233858327973No Hit
GTACATGGGAGCTCTGGGAGTGGAGCCCCAGCCTTGGGATTCCCAAGTGT8580.12158864492431178No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8410.1191795458990049No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC8180.11592017662947207No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8120.11506990638524611No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8030.11379450101890719No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7910.11209396053045526No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAGCCAC7730.1095431497977774No Hit
CCCTCATGCTGCCACCCCCGACTGTCACTGCACCGACCGGCCCTCGAGGA7720.10940143809040641No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7490.10614206882087357No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7470.10585864540613159No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG7450.10557522199138959No Hit
CCCCTGTGTTGGTCATCTATCAGAATACCAAGCGGCCCTCAGGGATCCCT7410.10500837516190563No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7370.10444152833242165No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7260.10288269955134074No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAT15350.056.772441
CAGCCGT16050.052.4791379
ATCAACG8150.051.250743
TCAACGC8400.050.582764
TATCAAC8550.049.695342
GTATCAA9000.049.614631
AATATAC17550.049.24183
TATACAG19200.046.5104145
ATACAGC20450.042.9540336
CAACGCA9900.042.91875
ATATACA21250.041.345734
AACGCAG10250.040.7419246
ACGCAGA10300.040.5441477
CGCAGAG10300.040.53848
ATAACGC907.781309E-540.008963
ATATTAC14050.037.4176333
TACTGTG14950.037.084037
TAATATA23600.036.9235232
GTATATT14050.036.907681
TACACCG1001.4469151E-436.0080645
Produced by FastQC (version 0.11.5)