FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689786
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTACCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTA11590.16802312601299532No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA10520.15251106865027705No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10150.14714708619774827No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8290.12018220143638751No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7420.10756959404800909No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18550.037.6613541
TCAACGC17400.037.2368054
ATCAACG17950.036.0958443
CAACGCA18050.035.8958665
AACGCAG18550.034.928326
TATCAAC19950.032.838072
CGCAGAG20550.031.52448
GTATAGG2253.0011506E-828.8089921
TATACGT1000.00628576528.7964614
ACGCAGA22750.028.475897
GTGCTAG2303.715286E-828.182711
GTACATG20850.026.9436631
GCAGAGT24550.026.6812449
ACATGGG20250.026.3078773
TACATGG21100.026.2716542
ATAGAGC2207.9545134E-726.1786023
CATGGGG13500.024.530324
GTATAAC2351.4081852E-624.5182911
TAGACTC1801.5939781E-423.9970515
GTCCTAC7850.023.8545821
Produced by FastQC (version 0.11.5)