FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689786
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTACCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTA11120.16120941857329665No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT9840.1426529387375215No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA8120.11771766895819864No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT7590.11003412652619798No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7450.10800451154416008No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7190.10423522657751826No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG24250.028.2231791
CTGTCCG1555.7654055E-527.869559
GTATAGA2603.974492E-927.7089921
TACATGG23950.027.3695012
CAGCGCT3451.1823431E-1025.0422049
CATGGGG13800.024.009234
ATTAGAC1801.5885002E-424.009233
ACATGGG26150.023.4124413
TCTATAC2802.4927795E-723.1517583
CTAGTAC1902.2913989E-422.7455883
TCAACGC16250.022.6056144
TATACTG3203.627065E-822.5070195
ATCAACG16950.022.096993
AACGCAG16850.021.7911766
CAACGCA16950.021.6704755
CTATAGG2003.237004E-421.6130141
GTATAGT3355.8720616E-821.5054861
GTCTAAG2353.892125E-521.459731
CTATACA2755.5099536E-620.9535124
GTATCAA18750.020.7484931
Produced by FastQC (version 0.11.5)