FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418498
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGATGGCCGAGTCCTCGGCTCTCAAGCTTCTCAGTTGCAGAGAGA17150.40979885208531464No Hit
AGCCTGGACAGTCCCTTAGACTCTCCTGTGCAGCCTCTGGATTCAGCTTC14090.33668022308350337No Hit
ATCTATTACTGTGCGAGACAAGTTAAATGCACTGGTGGTATATGCTATCT12340.29486401368704274No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9080.21696638932563594No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCAGCTTCAGTGACTTCTAC7850.1875755678641236No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7380.17634492876907418No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA7250.17323858178533708No Hit
GTCTGGGGGAGGCGTGGTCAAGCCTGGACAGTCCCTTAGACTCTCCTGTG7140.17061013433755956No Hit
ATACTACAGAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAACG6960.1663090385139236No Hit
ATATACTACAGAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAA6830.16320269153018652No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC6800.1624858422262472No Hit
GTAGTATATGGTCTCACCACTAGTGCTAATATATGAAATGCACTCCAGCC6300.15053835382725844No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6230.14886570545140002No Hit
CTCCAGGGACAACGCCAACAACTCTCTCTCTCTGCAACTGAGAAGCTTGA6170.14743200684352137No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG5890.14074141334008766No Hit
GTACATGGGAGTCCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT5770.13787401612433034No Hit
TATAAAAGGTGTCCAGTGTCAGGTGCAGCTGGTGCAGTCTGGGGGAGGCG5770.13787401612433034No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT5640.13476766914059327No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC5620.1342897696046337No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC5620.1342897696046337No Hit
GTCCAGGCTTGACCACGCCTCCCCCAGACTGCACCAGCTGCACCTGACAC5620.1342897696046337No Hit
CTACAGAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAACGCCA5590.1335729203006944No Hit
CTGTAGTATATGGTCTCACCACTAGTGCTAATATATGAAATGCACTCCAG5520.13190027192483594No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5250.12544862818938204No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT5040.12043068306180674No Hit
TATACCACCAGTGCATTTAACTTGTCTCGCACAGTAATAGATGGCCGAGT5020.11995278352584719No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT4660.11135059187857528No Hit
GTCCCTGGAGATGGTGAATCGGCCCTTCACAGAGTCTCTGTAGTATATGG4570.10920004396675731No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT4570.10920004396675731No Hit
GTATATGGTCTCACCACTAGTGCTAATATATGAAATGCACTCCAGCCCCT4470.10681054628695955No Hit
GTCTCTGTAGTATATGGTCTCACCACTAGTGCTAATATATGAAATGCACT4340.10370419930322249No Hit
GGTGGTATATGCTATCTTTCACTTGATTACTGGGGCCAGGGAGTCCTGGT4330.1034652495352427No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA4220.10083680208746518No Hit
CTCCTGTGCAGCCTCTGGATTCAGCTTCAGTGACTTCTACATGAGCTGGA4210.1005978523194854No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC4190.10011995278352585No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTACG451.2325631E-464.021481
GCGTGCG700.001092628641.1419039
GGGGAGT2252.1827873E-1135.199187
GTGTAGG2009.515134E-932.4108731
CTAAGCA900.003749039532.0030824
ATGGCCG8750.031.2678438
GTACACG2351.3533281E-930.6412495
TCTAAGC950.004881662330.3223323
TACATGG13550.030.2943632
GTACATG14100.030.137771
GTAATAG10050.030.099651
ACATGGG13900.030.0496523
GGGTACG2752.2919266E-1028.8096641
ATCAACG7750.028.806223
CCTATGG1000.006272583328.806223
TATGAGG1504.5981513E-528.8062172
TAAGGCT1753.9732968E-628.8027744
ATAGATG9850.028.510364
TCAACGC7900.028.2558884
GGACAGT12100.027.9662916
Produced by FastQC (version 0.11.5)