FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843182
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC69250.37570896417174215No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTG46490.252226855513997No Hit
GTCCTGGGCCCACTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC40990.22238715438844345No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38100.20670774779701626No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37720.20464609571925074No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAG36800.19965472753097632No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA36460.19781009146139666No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35940.19498888335498069No Hit
GGTTATAACTATGTCTCCTGGTACCAACAAACCCCAGGCAAAGCCCCCAA35860.194554851338609No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTT35220.19108259520763549No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT34070.1848433849722925No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA32930.17865842873899593No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT32440.17599998263871935No Hit
CTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATGTCTCCT31440.17057458243407325No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT29890.1621652121168718No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC28480.1545153978283208No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA27390.14860171160525656No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG27040.14670282153363043No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA26970.1463230435193052No Hit
GCTCATATACAAGCACCAACACTCTGGTGTTCGGCGGAGGGACCAAACTG26740.1450752014722366No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA26270.14252526337605295No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26000.1410604053207985No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25710.13948703926145112No Hit
TCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCACTCTGCCCTG25220.13682859316117454No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25150.1364488151468493No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24860.13487544908750193No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG24690.13395313105271212No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG24320.13194573297699305No Hit
GTATATGAGCTGCAGAAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA24180.1311861769483426No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG24050.1304808749217386No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT23320.12652033277234695No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCACTC22900.1242416646863956No Hit
TCCTCACTCAGGTCACAGGGTCCTGGGCCCACTCTGCCCTGACTCAGCCT22680.12304807664137345No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTGC21780.11816521645719197No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21620.11729715242444858No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATA20980.1138248962934751No Hit
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTAGCAGATACA20540.1114377202034308No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT20500.11122070419524496No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA20040.10872502010110777No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19710.10693463803357454No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCT19170.10400492192306565No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA19160.10395066792101919No Hit
TATATGAGCTGCAGAAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAG19110.10367939791078688No Hit
GTGTATTACTGTGCGAAAGATCTCCGATACAGTTATGGTTACGGGGCTAT18850.10226879385757891No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)