FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7309
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA370.5062252018059926No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA320.43781639075112877No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA270.36940757969626487No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC250.34204405527431936No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA190.2599534820084827No Hit
CACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGC130.17786290874264604No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC120.1641811465316733No Hit
CCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAC120.1641811465316733No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT110.1504993843207005No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC110.1504993843207005No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA110.1504993843207005No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC100.13681762210972773No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA90.12313585989875496No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT90.12313585989875496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.12313585989875496No Hit
TCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGG80.10945409768778219No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG80.10945409768778219No Hit
GCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATC80.10945409768778219No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG151.21398596E-4144.000023
CAACGCA151.21398596E-4144.000025
AAGAGAG100.007093595144.05
ACATACT100.007093595144.04
AGACATA100.007093595144.02
CCTAGGC100.007093595144.03
AACGCAG203.8161338E-4108.06
ACGCAGA203.8161338E-4108.07
GTATCAA203.8161338E-4108.01
TCAACGC203.8161338E-4108.04
TATCAAC203.8161338E-4108.02
GCAGAGT300.001911171472.000019
CGCAGAG300.001911171472.000018
CCGCTAC350.00352162561.7142839
TAATAAA400.00597540754.01
AAAAAAA650.00816435313.292308130-134
Produced by FastQC (version 0.11.5)