FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788259
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATTACTGTGCGAGAGGGGGGGATGGCTATTCTCTTGACAATTGGGG35620.19918814892026268No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35180.19672765522220215No Hit
GTAATAGATAGCCGTGTCCTCGACTCTCAGTCTGCCCATTTGCAAATACA30520.1706687901472885No Hit
GTTTAATAATTACCTGGTCACCTTCGGCCAAGGGACACGACTGGAGATTA25230.14108694545924277No Hit
TTATTAAACTGTTGACAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCT24930.13940933611965603No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24000.13420874716693723No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20170.11279126793154683No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36600.079.0818561
ATCAACG43450.066.290413
TCAACGC43550.065.642154
CGCAGAG56250.051.0776448
CAACGCA56150.051.0403675
AACGCAG58350.049.3627786
ACGCAGA59750.048.0856487
TATCAAC62950.046.6707652
GCAGAGT65200.043.6146979
GGTATCA31700.035.6592561
GTACATG45050.031.165331
GAATACG3500.030.853659
TACATGG45450.030.41922
TAGATAG16700.030.1829765
GTAATAG18050.029.5179631
CTGTGCG20200.028.8680179
ACATGGG47400.028.8639493
GATAGCC17100.028.2136427
CATGGGA20850.027.283594
TAATAGA18500.027.2462542
Produced by FastQC (version 0.11.5)