FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636380
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT55310.33800217553380024No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42610.2603918405260392No Hit
GTAATAAAAGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG40480.24737530402473754No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCACT35690.21810337452181036No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34540.21107566702110755No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCTTTTATTA34320.20973123602097316No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29920.18284261601828428No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA29420.1797870910179787No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG29310.17911487551791147No Hit
GAGTTACAGTGGCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC29100.17783155501778317No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA29090.17777044451777704No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28990.17715933951771592No Hit
ACTTTGCACAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGAC28170.17214827851721484No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC27660.16903164301690315No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26910.16444835551644485No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG26870.16420391351642039No Hit
GACCAGGGAAAGCCCCTAACCTCCTGATCTATGATGCATCCACTTTGCAC26360.16108727801610873No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25900.15827619501582763No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25780.1575428690157543No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25370.15503733851550372No Hit
TCCCTATCTTTGGTACAGCAAACTACGCGCAGAAGTTCCAGGGCAGAGTC25140.1536317970153632No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24160.1476429680147643No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23980.1465429790146543No Hit
GTGCAAAGTGGATGCATCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTC23460.1433652330143365No Hit
CTTTTATTACTGTGCGAGAGATTGTAGTCCTACCACCTGCTATCGAGACT23440.1432430120143243No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCTGAGCATTAGCAG23400.14299857001429986No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG23030.14073748151407375No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT23030.14073748151407375No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22620.13823195101382318No Hit
GATCTATGATGCATCCACTTTGCACAGTGGGGTCCCATCAAGGTTCAGTG22590.13804861951380484No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22420.13700974101370098No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22010.13450421051345043No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT21960.13419865801341985No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT21830.13340422151334042No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21480.13126535401312656No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT20780.12698761901269875No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20720.1266209560126621No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.12393209401239322No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20100.12283210501228323No Hit
CATAACAACCACATCCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT19210.11739327051173933No Hit
ACTTACTACTGTCAACAGAGTTACAGTGGCCGGACGTTCGGCCAAGGGAC19170.11714882851171489No Hit
GTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCACTGTGCAAAGT19100.1167210550116721No Hit
CCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCC18630.1138488615113849No Hit
CATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCCT18530.11323775651132378No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18450.11274887251127488No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA18190.11115999951111599No Hit
TCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTCTCTGCTGAAACCAATT17930.10957112651095711No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17870.10920446351092045No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC17810.10883780051088376No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCTGA17790.10871557951087156No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17780.10865446901086545No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17380.106210049010621No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC17220.10523228101052323No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA17110.104560065510456No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17070.10431562351043155No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17030.10407118151040712No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16930.10346007651034601No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA16900.10327674501032767No Hit
TTTTATTACTGTGCGAGAGATTGTAGTCCTACCACCTGCTATCGAGACTG16690.10199342451019935No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)